MicroRNAs shape circadian hepatic gene expression on a transcriptome-wide scale.

Détails

Ressource 1Télécharger: BIB_9EC4C39CC571.P001.pdf (5440.46 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_9EC4C39CC571
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
MicroRNAs shape circadian hepatic gene expression on a transcriptome-wide scale.
Périodique
Elife
Auteur(s)
Du N.H., Arpat A.B., De Matos M., Gatfield D.
ISSN
2050-084X (Electronic)
Statut éditorial
Publié
Date de publication
2014
Volume
3
Pages
e02510
Langue
anglais
Résumé
A considerable proportion of mammalian gene expression undergoes circadian oscillations. Post-transcriptional mechanisms likely make important contributions to mRNA abundance rhythms. We have investigated how microRNAs (miRNAs) contribute to core clock and clock-controlled gene expression using mice in which miRNA biogenesis can be inactivated in the liver. While the hepatic core clock was surprisingly resilient to miRNA loss, whole transcriptome sequencing uncovered widespread effects on clock output gene expression. Cyclic transcription paired with miRNA-mediated regulation was thus identified as a frequent phenomenon that affected up to 30% of the rhythmic transcriptome and served to post-transcriptionally adjust the phases and amplitudes of rhythmic mRNA accumulation. However, only few mRNA rhythms were actually generated by miRNAs. Overall, our study suggests that miRNAs function to adapt clock-driven gene expression to tissue-specific requirements. Finally, we pinpoint several miRNAs predicted to act as modulators of rhythmic transcripts, and identify rhythmic pathways particularly prone to miRNA regulation.DOI: http://dx.doi.org/10.7554/eLife.02510.001.
Pubmed
Web of science
Création de la notice
24/06/2014 16:19
Dernière modification de la notice
20/08/2019 16:04
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