Absence of rhodamine 123-photochemotoxicity in human tumor xenografts
Details
Serval ID
serval:BIB_9E756BB8B0B4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Absence of rhodamine 123-photochemotoxicity in human tumor xenografts
Journal
Lasers in Surgery and Medicine
ISSN
0196-8092 (Print)
Publication state
Published
Issued date
1993
Volume
13
Number
1
Pages
40-4
Notes
Journal Article --- Old url value: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8426526
Abstract
Rhodamine 123 (R123)-photochemotoxicity was assessed in BALB/c nude mice bearing a xenografted human squamous cell carcinoma at various power densities and wavelengths and a given incident fluence of 150 Joules/cm2. One hour before light delivery, 1 mg R123/kg was injected i.p. in 20 animals. Surface irradiance was performed on the tumor and an equal size hind leg area of 40 animals. Three groups of 10 animals were treated at 514.5 nm and 0.1 W/cm2, 1 W/cm2, and 30 W/cm2, and one at 488 nm and 30 W/cm2. In each group, five animals received R123. The R123 concentration was measured in the tumor (0.023 +/- 0.007 micrograms/g) and tumor-free tissue (0.023 +/- 0.008 micrograms/g) in three additional animals by high performance liquid chromatography 1 hour after R123-administration. Histologic assessment 72 hours after light delivery revealed no tissue damage at nonthermal power densities, either in the tumor or in the tumor-free tissue, irrespective of R123-administration. At 30 W/cm2, neither in the tumor nor in tumor-free tissues was there any significant difference in the depth of necrosis, irrespective of R123-administration and the wavelength applied. Our results question the validity of R123 as a photosensitizer, at least in this rodent tumor model.
Keywords
Animals
Carcinoma, Squamous Cell/chemistry/*drug therapy/pathology
Chromatography, High Pressure Liquid
Fluorescent Dyes/analysis/*pharmacology
Heat/adverse effects
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
Muscles/drug effects/pathology
Necrosis
Neoplasm Transplantation
*Photochemotherapy
Rhodamine 123
Rhodamines/analysis/*pharmacology
Skin/drug effects/pathology
Pubmed
Web of science
Create date
29/01/2008 14:00
Last modification date
20/08/2019 16:04