The effect of beef spleen cathepsin-D on pig heart lactate-dehydrogenase

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_9E4E538C31CA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The effect of beef spleen cathepsin-D on pig heart lactate-dehydrogenase
Périodique
Archives of Biochemistry and Biophysics
Auteur(s)
Widmer F., Widmer C.
ISSN
0003-9861
Statut éditorial
Publié
Date de publication
1975
Peer-reviewed
Oui
Volume
168
Numéro
1
Pages
252-258
Langue
anglais
Résumé
Pig heart lactate dehydrogenase (EC 1.1.1.27; H4 isoenzyme) has been subjected to the action of beef spleen cathepsin D (EC 3.4.23.5) at pH 3.9. No proteolytic effect on the tetrameric form of the dehydrogenase could be detected. On the other hand, the acid proteinase appears to act on the dissociated monomers in such a manner that they are unable to reassociate into functional tetramers. This finding could be correlated with proteolytic modification of the monomers. The extent of proteolysis depends on the temperature and duration of the incubation.
Mots-clé
Animals, *Cathepsins, Cattle, Chromatography, Affinity, Chromatography, Gel, Isoenzymes, Kinetics, L-Lactate Dehydrogenase/*metabolism, Macromolecular Substances, Myocardium/*enzymology, NAD/analogs & derivatives, Protein Binding, Protein Denaturation, Spleen/*enzymology, Swine
Pubmed
Web of science
Création de la notice
13/08/2015 7:53
Dernière modification de la notice
20/08/2019 15:04
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