Fas ligand-induced c-Jun kinase activation in lymphoid cells requires extensive receptor aggregation but is independent of DAXX, and Fas-mediated cell death does not involve DAXX, RIP, or RAIDD
Details
Serval ID
serval:BIB_9E45021E2C74
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Fas ligand-induced c-Jun kinase activation in lymphoid cells requires extensive receptor aggregation but is independent of DAXX, and Fas-mediated cell death does not involve DAXX, RIP, or RAIDD
Journal
Journal of Immunology
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
08/2000
Volume
165
Number
3
Pages
1337-43
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 1
Research Support, Non-U.S. Gov't --- Old month value: Aug 1
Abstract
Jun kinase signaling can be elicited by death receptor activation, but the mechanism and significance of this event are still unclear. It has been reported that cross-linking Abs to Fas trigger c-Jun N-terminal kinase (JNK) signaling via caspase-mediated activation of MEKK1 (JNK kinase kinase), elevation of ceramide levels or by recruitment of death domain associated protein (DAXX) to Fas. The effect of physiological ligand for Fas on JNK signaling was never investigated, although evidence is accumulating that Fas ligand is able to induce cellular responses distinct from those evoked by Ab-mediated cross-linking of Fas. Therefore, we investigated the effect of Fas ligand on JNK signaling. Like its ability to induce cell death, Fas ligand reliably activated JNK only upon extensive aggregation of the receptor. Although this was partially dependent on caspase activation, DAXX was not required. DAXX and other death receptor-associated proteins, which have been reported to bind directly or indirectly to Fas, such as receptor interacting protein (RIP) and RIP-associated ICH-1/CED-3-homologous protein with a death domain (RAIDD), were shown to be dispensable for Fas ligand-induced apoptosis.
Keywords
Adaptor Proteins, Signal Transducing
Animals
Antigens, CD95/immunology/metabolism/*physiology
Apoptosis/genetics/*immunology
Carrier Proteins/biosynthesis/genetics/*physiology
Caspases/physiology
Cell Line, Transformed
Enzyme Activation/immunology
Fas Ligand Protein
Genetic Vectors/chemical synthesis/pharmacology
Humans
*Intracellular Signaling Peptides and Proteins
JNK Mitogen-Activated Protein Kinases
Jurkat Cells
Ligands
Lymphocytes/cytology/*enzymology/immunology/metabolism
Membrane Glycoproteins/immunology/metabolism/*physiology
Mice
Mitogen-Activated Protein Kinases/*metabolism
*Nuclear Proteins
Protein Biosynthesis
Proteins/genetics/*physiology
Receptor Aggregation/*immunology
Receptor-Interacting Protein Serine-Threonine Kinases
Signal Transduction/genetics/immunology
Tumor Cells, Cultured
Pubmed
Web of science
Create date
24/01/2008 16:18
Last modification date
20/08/2019 16:04