Amine-reactive OVA multimers for auto-vaccination against cytokines and other mediators: perspectives illustrated for GCP-2 in L. major infection.

Détails

ID Serval
serval:BIB_9E11DAC7E432
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Amine-reactive OVA multimers for auto-vaccination against cytokines and other mediators: perspectives illustrated for GCP-2 in L. major infection.
Périodique
Journal of Leukocyte Biology
Auteur(s)
Uyttenhove C., Marillier R.G., Tacchini-Cottier F., Charmoy M., Caspi R.R., Damsker J.M., Goriely S., Su D., Van Damme J., Struyf S., Opdenakker G., Van Snick J.
ISSN
1938-3673 (Electronic)
ISSN-L
0741-5400
Statut éditorial
Publié
Date de publication
2011
Volume
89
Numéro
6
Pages
1001-1007
Langue
anglais
Résumé
Anticytokine auto-vaccination is a powerful tool for the study of cytokine functions in vivo but has remained rather esoteric as a result of numerous technical difficulties. We here describe a two-step procedure based on the use of OVA multimers purified by size exclusion chromatography after incubation with glutaraldehyde at pH 6. When such polymers are incubated with a target protein at pH 8.5 to deprotonate reactive amines, complexes are formed that confer immunogenicity to self-antigens. The chemokine GCP-2/CXCL6, the cytokines GM-CSF, IL-17F, IL-17E/IL-25, IL-27, and TGF-β1, and the MMP-9/gelatinase B are discussed as examples. mAb, derived from such immunized mice, have obvious advantages for in vivo studies of the target proteins. Using a mAb against GCP-2, obtained by the method described here, we provide the first demonstration of the major role played by this chemokine in rapid neutrophil mobilization after Leishmania major infection. Pre-activated OVA multimers reactive with amine residues thus provide an efficient carrier for auto-vaccination against 9-90 kDa autologous proteins.
Mots-clé
Amines/chemistry, Animals, Cell Movement, Chemokines/immunology, Cytokines/immunology, Glutaral/metabolism, Immunization, Interleukin-17/physiology, Leishmania major/immunology, Leishmaniasis, Cutaneous/immunology, Leishmaniasis, Cutaneous/parasitology, Mice, Mice, Inbred C57BL, Mice, Knockout, Microtubule-Associated Proteins/immunology, Neutrophils, Ovalbumin/immunology, Protein Multimerization
Pubmed
Web of science
Création de la notice
27/10/2011 14:15
Dernière modification de la notice
20/08/2019 16:04
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