Article: article from journal or magazin.
Activation of influenza virus-specific CD4+ and CD8+ T cells: a new role for plasmacytoid dendritic cells in adaptive immunity.
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Plasmacytoid dendritic cells (pDCs) contribute to innate antiviral immune responses by producing type I interferons (IFNs) upon exposure to enveloped viruses. However, their role in adaptive immune responses, such as the initiation of antiviral T-cell responses, is not known. In this study, we examined interactions between blood pDCs and influenza virus with special attention to the capacity of pDCs to activate influenza-specific T cells. pDCs were compared with CD11c(+) DCs, the most potent antigen-presenting cells (APCs), for their capacity to activate T-cell responses. We found that like CD11c(+) DCs, pDCs mature following exposure to influenza virus, express CCR7, and produce proinflammatory chemokines, but differ in that they produce type I IFN and are resistant to the cytopathic effect of the infection. After influenza virus exposure, both DC types exhibited an equivalent efficiency to expand anti-influenza virus cytotoxic T lymphocytes (CTLs) and T helper 1 (TH1) CD4(+) T cells. Our results pinpoint a new role of pDCs in the induction of antiviral T-cell responses and suggest that these DCs play a prominent role in the adaptive immune response against viruses.
Antigen Presentation, Antigens, CD11c/analysis, Antigens, Viral/immunology, Antigens, Viral/physiology, Cells, Cultured/immunology, Cells, Cultured/metabolism, Chemokines/biosynthesis, Cytopathogenic Effect, Viral, Dendritic Cells/classification, Dendritic Cells/immunology, HLA-A2 Antigen/immunology, Humans, Immunologic Memory/immunology, Influenza A virus/immunology, Interferon-alpha/biosynthesis, Lymphocyte Activation/immunology, Peptide Fragments/immunology, Receptors, CCR7, Receptors, Chemokine/biosynthesis, T-Lymphocytes, Cytotoxic/immunology, Th1 Cells/immunology, Viral Matrix Proteins/immunology
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