Inactivation of the Anti-Müllerian Hormone Receptor Type 2 (amhrII) Gene in Northern Pike (Esox lucius) Results in Male-To-Female Sex Reversal.
Details
Serval ID
serval:BIB_9D46CFCAC01A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Inactivation of the Anti-Müllerian Hormone Receptor Type 2 (amhrII) Gene in Northern Pike (Esox lucius) Results in Male-To-Female Sex Reversal.
Journal
Sexual development
ISSN
1661-5433 (Electronic)
ISSN-L
1661-5425
Publication state
Published
Issued date
2022
Peer-reviewed
Oui
Volume
16
Number
4
Pages
289-294
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
The anti-müllerian hormone (Amh) pathway is crucial for sexual development in teleosts. A male-specific duplicate of anti-müllerian hormone (amhby) was previously identified as the northern pike (Esox lucius) master sex determination gene. However, the role of its putative cognate receptor, i.e., the anti-müllerian hormone receptor type 2 (amhrII) was unclear in this species.
Here, we investigated the role of amhrII during sexual development of northern pike.
We generated stable mutants with deletions in exon 9 of amhrII, inactivating the AmhrII protein using a CRISPR-Cas9-mediated gene knockout strategy.
The inactivation of amhrII in northern pike results in a high level of male-to-female sex reversal.
This result demonstrates that amhrII is necessary for male sexual development in northern pike and supports the idea that AmhrII is a conserved regulator of the teleosts sex differentiation network.
Here, we investigated the role of amhrII during sexual development of northern pike.
We generated stable mutants with deletions in exon 9 of amhrII, inactivating the AmhrII protein using a CRISPR-Cas9-mediated gene knockout strategy.
The inactivation of amhrII in northern pike results in a high level of male-to-female sex reversal.
This result demonstrates that amhrII is necessary for male sexual development in northern pike and supports the idea that AmhrII is a conserved regulator of the teleosts sex differentiation network.
Keywords
Animals, Male, Female, Anti-Mullerian Hormone/genetics, Anti-Mullerian Hormone/metabolism, Esocidae/metabolism, Receptors, Peptide/genetics, Receptors, Transforming Growth Factor beta/genetics, AMHRII, CRISPR-Cas9, Fish Gene inactivation, Sex determination
Pubmed
Web of science
Create date
22/03/2022 0:29
Last modification date
28/02/2023 7:51
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