Long-term treatment of aggregating brain cell cultures with low concentrations of lead acetate.

Details

Serval ID
serval:BIB_9C9F41A9F94D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Long-term treatment of aggregating brain cell cultures with low concentrations of lead acetate.
Journal
Neurotoxicology
Author(s)
Zurich M.G., Monnet-Tschudi F., Honegger P.
ISSN
0161-813X[print], 0161-813X[linking]
Publication state
Published
Issued date
1994
Volume
15
Number
3
Pages
715-719
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Aggregating brain cell cultures were used as a model to study the effect of chronic exposure to low levels of lead acetate. Long-term maintenance of cultures could be improved by supplementation of the medium with albumin-bound lipids. Exposure for 9 days to 10(-6)-10(-4) M lead acetate caused a decrease of GABAergic (glutamic acid decarboxylase) and astrocytic (glutamine synthetase) markers which was also found after prolonged treatment (50 days) with 10(-7) M lead acetate. Total protein content and choline acetyltransferase were not changed. The results show that prolonged exposure of aggregating brain cell cultures to a low concentration of lead acetate causes distinct changes of cell type-specific parameters.
Keywords
Albumins/pharmacology, Animals, Brain/drug effects, Brain/enzymology, Cell Aggregation, Cells, Cultured, Choline O-Acetyltransferase/metabolism, Glutamate Decarboxylase/metabolism, Glutamate-Ammonia Ligase/metabolism, Lipids/pharmacology, Organometallic Compounds/toxicity, Rats
Pubmed
Web of science
Create date
24/01/2008 13:12
Last modification date
20/08/2019 15:03
Usage data