Human ERK1 induces filamentous growth and cell wall remodeling pathways in Saccharomyces cerevisiae.

Détails

ID Serval
serval:BIB_9C8718F2D753
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Human ERK1 induces filamentous growth and cell wall remodeling pathways in Saccharomyces cerevisiae.
Périodique
Journal of Biological Chemistry
Auteur(s)
Atienza J.M., Suh M., Xenarios I., Landgraf R., Colicelli J.
ISSN
0021-9258 (Print)
ISSN-L
0021-9258
Statut éditorial
Publié
Date de publication
2000
Volume
275
Numéro
27
Pages
20638-20646
Langue
anglais
Résumé
Expression of an activated extracellular signal-regulated kinase 1 (ERK1) construct in yeast cells was used to examine the conservation of function among mitogen-activated protein (MAP) kinases. Sequence alignment of the human MAP kinase ERK1 with all Saccharomyces cerevisiae kinases reveals a particularly strong kinship with Kss1p (invasive growth promoting MAP kinase), Fus3p (pheromone response MAP/ERK kinase), and Mpk1p (cell wall remodeling MAP kinase). A fusion protein of constitutively active human MAP/ERK kinase 1 (MEK) and human ERK1 was introduced under regulated expression into yeast cells. The fusion protein (MEK/ERK) induced a filamentation response element promoter and led to a growth retardation effect concomitant with a morphological change resulting in elongated cells, bipolar budding, and multicell chains. Induction of filamentous growth was also observed for diploid cells following MEK/ERK expression in liquid culture. Neither haploids nor diploids, however, showed marked penetration of agar medium. These effects could be triggered by either moderate MEK/ERK expression at 37 degrees C or by high level MEK/ERK expression at 30 degrees C. The combination of high level MEK/ERK expression and 37 degrees C resulted in cell death. The deleterious effects of MEK/ERK expression and high temperature were significantly mitigated by 1 m sorbitol, which also enhanced the filamentous phenotype. MEK/ERK was able to constitutively activate a cell wall maintenance reporter gene, suggesting misregulation of this pathway. In contrast, MEK/ERK effectively blocked expression from a pheromone-responsive element promoter and inhibited mating. These results are consistent with MEK/ERK promoting filamentous growth and altering the cell wall through its ability to partially mimic Kss1p and stimulate a pathway normally controlled by Mpk1p, while appearing to inhibit the normal functioning of the structurally related yeast MAP kinase Fus3p.
Mots-clé
Cell Division/genetics, Cell Wall/metabolism, Fungal Proteins/metabolism, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Fungal, Genes, Reporter, Humans, Mitogen-Activated Protein Kinase 3, Mitogen-Activated Protein Kinases/metabolism, Phenotype, Recombinant Fusion Proteins/metabolism, Saccharomyces cerevisiae/enzymology, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins, Sequence Alignment, Sorbitol/pharmacology
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/10/2012 9:16
Dernière modification de la notice
20/08/2019 15:03
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