Sodium-dependent phosphate transporters in osteoclast differentiation and function.

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Ressource 1Télécharger: BIB_9C533E1C9579.P001.pdf (2625.39 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_9C533E1C9579
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Sodium-dependent phosphate transporters in osteoclast differentiation and function.
Périodique
Plos One
Auteur(s)
Albano G., Moor M., Dolder S., Siegrist M., Wagner C.A., Biber J., Hernando N., Hofstetter W., Bonny O., Fuster D.G.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
04/2015
Peer-reviewed
Oui
Volume
10
Numéro
4
Pages
e0125104
Langue
anglais
Résumé
Osteoclasts are multinucleated bone degrading cells. Phosphate is an important constituent of mineralized bone and released in significant quantities during bone resorption. Molecular contributors to phosphate transport during the resorptive activity of osteoclasts have been controversially discussed. This study aimed at deciphering the role of sodium-dependent phosphate transporters during osteoclast differentiation and bone resorption. Our studies reveal RANKL-induced differential expression of sodium-dependent phosphate transport protein IIa (NaPi-IIa) transcript and protein during osteoclast development, but no expression of the closely related NaPi-IIb and NaPi-IIc SLC34 family isoforms. In vitro studies employing NaPi-IIa-deficient osteoclast precursors and mature osteoclasts reveal that NaPi-IIa is dispensable for bone resorption and osteoclast differentiation. These results are supported by the analysis of structural bone parameters by high-resolution microcomputed tomography that yielded no differences between adult NaPi-IIa WT and KO mice. By contrast, both type III sodium-dependent phosphate transporters Pit-1 and Pit-2 were abundantly expressed throughout osteoclast differentiation, indicating that they are the relevant sodium-dependent phosphate transporters in osteoclasts and osteoclast precursors. We conclude that phosphate transporters of the SLC34 family have no role in osteoclast differentiation and function and propose that Pit-dependent phosphate transport could be pivotal for bone resorption and should be addressed in further studies.
Pubmed
Web of science
Open Access
Oui
Création de la notice
14/01/2016 9:38
Dernière modification de la notice
20/08/2019 15:03
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