The functions of DNA methylation by CcrM in Caulobacter crescentus: a global approach.

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Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_9C1AC2294C78
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The functions of DNA methylation by CcrM in Caulobacter crescentus: a global approach.
Périodique
Nucleic Acids Research
Auteur(s)
Gonzalez D., Kozdon J.B., McAdams H.H., Shapiro L., Collier J.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
42
Numéro
6
Pages
3720-3735
Langue
anglais
Résumé
DNA methylation is involved in a diversity of processes in bacteria, including maintenance of genome integrity and regulation of gene expression. Here, using Caulobacter crescentus as a model, we exploit genome-wide experimental methods to uncover the functions of CcrM, a DNA methyltransferase conserved in most Alphaproteobacteria. Using single molecule sequencing, we provide evidence that most CcrM target motifs (GANTC) switch from a fully methylated to a hemi-methylated state when they are replicated, and back to a fully methylated state at the onset of cell division. We show that DNA methylation by CcrM is not required for the control of the initiation of chromosome replication or for DNA mismatch repair. By contrast, our transcriptome analysis shows that >10% of the genes are misexpressed in cells lacking or constitutively over-expressing CcrM. Strikingly, GANTC methylation is needed for the efficient transcription of dozens of genes that are essential for cell cycle progression, in particular for DNA metabolism and cell division. Many of them are controlled by promoters methylated by CcrM and co-regulated by other global cell cycle regulators, demonstrating an extensive cross talk between DNA methylation and the complex regulatory network that controls the cell cycle of C. crescentus and, presumably, of many other Alphaproteobacteria.
Mots-clé
Alphaproteobacteria/genetics, Bacterial Proteins/classification, Bacterial Proteins/metabolism, Caulobacter crescentus/enzymology, Caulobacter crescentus/genetics, Chromosomes, Bacterial, DNA Methylation, Gene Expression Regulation, Bacterial, Genome, Bacterial, Mutation Rate, Nucleotide Motifs, Phylogeny, Site-Specific DNA-Methyltransferase (Adenine-Specific)/classification, Site-Specific DNA-Methyltransferase (Adenine-Specific)/metabolism, Transcriptome
Pubmed
Web of science
Open Access
Oui
Création de la notice
06/12/2013 12:19
Dernière modification de la notice
08/05/2019 22:40
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