Conservation tillage and organic farming induce minor variations in Pseudomonas abundance, their antimicrobial function and soil disease resistance.

Détails

Ressource 1Télécharger: Dennert-FEMSMIcrobiolEcol-2018 OA voie verte.pdf (2986.79 [Ko])
Etat: Serval
Version: Author's accepted manuscript
Licence: Non spécifiée
ID Serval
serval:BIB_9C15D6ABCD0D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Conservation tillage and organic farming induce minor variations in Pseudomonas abundance, their antimicrobial function and soil disease resistance.
Périodique
FEMS microbiology ecology
Auteur(s)
Dennert F., Imperiali N., Staub C., Schneider J., Laessle T., Zhang T., Wittwer R., van der Heijden MGA, Smits THM, Schlaeppi K., Keel C., Maurhofer M.
ISSN
1574-6941 (Electronic)
ISSN-L
0168-6496
Statut éditorial
Publié
Date de publication
01/08/2018
Peer-reviewed
Oui
Volume
94
Numéro
8
Pages
1
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Conservation tillage and organic farming are strategies used worldwide to preserve the stability and fertility of soils. While positive effects on soil structure have been extensively reported, the effects on specific root- and soil-associated microorganisms are less known. The aim of this study was to investigate how conservation tillage and organic farming influence the frequency and activity of plant-beneficial pseudomonads. Amplicon sequencing using the 16S rRNA gene revealed that Pseudomonas is among the most abundant bacterial taxa in the root microbiome of field-grown wheat, independent of agronomical practices. However, pseudomonads carrying genes required for the biosynthesis of specific antimicrobial compounds were enriched in samples from conventionally farmed plots without tillage. In contrast, disease resistance tests indicated that soil from conventional no tillage plots is less resistant to the soilborne pathogen Pythium ultimum compared to soil from organic reduced tillage plots, which exhibited the highest resistance of all compared cropping systems. Reporter strain-based gene expression assays did not reveal any differences in Pseudomonas antimicrobial gene expression between soils from different cropping systems. Our results suggest that plant-beneficial pseudomonads can be favoured by certain soil cropping systems, but soil resistance against plant diseases is likely determined by a multitude of biotic factors in addition to Pseudomonas.
Mots-clé
Antibiosis/physiology, Antiparasitic Agents/metabolism, Disease Resistance/physiology, Farms, Microbiota, Organic Agriculture/methods, Plant Diseases/parasitology, Plant Diseases/prevention & control, Plant Roots/microbiology, Pseudomonas/genetics, Pseudomonas/isolation & purification, Pseudomonas/metabolism, Pythium/drug effects, RNA, Ribosomal, 16S/genetics, Soil/chemistry, Soil Microbiology, Triticum/microbiology, Triticum/parasitology
Pubmed
Web of science
Création de la notice
03/05/2018 18:47
Dernière modification de la notice
27/05/2019 6:11
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