Transcriptional interference by RNA polymerase III affects expression of the Polr3e gene.

Détails

Ressource 1Télécharger: Genes Dev.-2017-Yeganeh-413-21.pdf (1057.75 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_9C06585B158C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Transcriptional interference by RNA polymerase III affects expression of the Polr3e gene.
Périodique
Genes and Development
Auteur(s)
Yeganeh M., Praz V., Cousin P., Hernandez N.
ISSN
1549-5477 (Electronic)
ISSN-L
0890-9369
Statut éditorial
Publié
Date de publication
2017
Peer-reviewed
Oui
Volume
31
Numéro
4
Pages
413-421
Langue
anglais
Résumé
Overlapping gene arrangements can potentially contribute to gene expression regulation. A mammalian interspersed repeat (MIR) nested in antisense orientation within the first intron of the Polr3e gene, encoding an RNA polymerase III (Pol III) subunit, is conserved in mammals and highly occupied by Pol III. Using a fluorescence assay, CRISPR/Cas9-mediated deletion of the MIR in mouse embryonic stem cells, and chromatin immunoprecipitation assays, we show that the MIR affects Polr3e expression through transcriptional interference. Our study reveals a mechanism by which a Pol II gene can be regulated at the transcription elongation level by transcription of an embedded antisense Pol III gene.

Mots-clé
RNA polymerase, gene expression regulation, Polr3e gene, antisense transcription, transcriptional interference, SINE
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/03/2017 19:06
Dernière modification de la notice
20/08/2019 16:02
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