Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based trafficking.

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Ressource 1Télécharger: BIB_9B4198FCF94C.P001.pdf (8988.60 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_9B4198FCF94C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based trafficking.
Périodique
PLoS Biology
Auteur(s)
Bendezú F.O., Vincenzetti V., Vavylonis D., Wyss R., Vogel H., Martin S.G.
ISSN
1545-7885 (Electronic)
ISSN-L
1544-9173
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
13
Numéro
4
Pages
e1002097
Langue
anglais
Résumé
The small Rho-family GTPase Cdc42 is critical for cell polarization and polarizes spontaneously in absence of upstream spatial cues. Spontaneous polarization is thought to require dynamic Cdc42 recycling through Guanine nucleotide Dissociation Inhibitor (GDI)-mediated membrane extraction and vesicle trafficking. Here, we describe a functional fluorescent Cdc42 allele in fission yeast, which demonstrates Cdc42 dynamics and polarization independent of these pathways. Furthermore, an engineered Cdc42 allele targeted to the membrane independently of these recycling pathways by an amphipathic helix is viable and polarizes spontaneously to multiple sites in fission and budding yeasts. We show that Cdc42 is highly mobile at the membrane and accumulates at sites of activity, where it displays slower mobility. By contrast, a near-immobile transmembrane domain-containing Cdc42 allele supports viability and polarized activity, but does not accumulate at sites of activity. We propose that Cdc42 activation, enhanced by positive feedback, leads to its local accumulation by capture of fast-diffusing inactive molecules.
Mots-clé
Actins/metabolism, Alleles, Cell Polarity, Fluorescent Dyes, Guanine Nucleotide Dissociation Inhibitors/metabolism, Protein Transport, Schizosaccharomyces/cytology, Schizosaccharomyces/metabolism, cdc42 GTP-Binding Protein/genetics, cdc42 GTP-Binding Protein/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
12/06/2015 17:48
Dernière modification de la notice
08/05/2019 22:37
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