Genetic and physiological characterization of Pseudomonas aeruginosa mutants affected in the catabolic ornithine carbamoyltransferase.

Détails

ID Serval
serval:BIB_9AE759E0040B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Genetic and physiological characterization of Pseudomonas aeruginosa mutants affected in the catabolic ornithine carbamoyltransferase.
Périodique
Journal of Bacteriology
Auteur(s)
Hass D., Evans R., Mercenier A., Simon J.P., Stalon V.
ISSN
0021-9193 (Print)
ISSN-L
0021-9193
Statut éditorial
Publié
Date de publication
1979
Volume
139
Numéro
3
Pages
713-720
Langue
anglais
Résumé
In Pseudomonas aeruginosa arginine can be degraded by the arginine "dihydrolase" system, consisting of arginine deiminase, catabolic ornithine carbamoyltransferase, and carbamate kinase. Mutants of P. aeruginosa strain PAO affected in the structural gene (arcB) of the catabolic ornithine carbamoyltransferase were isolated. Firt, and argF mutation (i.e., a block in the anabolic ornithine carbamoyltransferase) was suppressed specifically by a mutationally altered catabolic ornithine carbamoyltransferase capable of functioning in the anabolic direction. The suppressor locus arcB (Su) was mapped by transduction between hisII and argA. Second, mutants having lost suppressor activity were obtained. The Su- mutations were very closely linked to arcB (Su) and caused strongly reduced ornithine carbamoyltransferase activities in vitro. Under aerobic conditions, a mutant (PA0630) which had less than 1% of the wild-type catabolic ornithine carbamoyltransferase activity grew on arginine as the only carbon and nitrogen source, at the wild-type growth rate. When oxygen was limiting, strain PA0630 grown on arginine excreted citrulline in the stationary growth phase. These observations suggest that during aerobic growth arginine is not degraded exclusively via the dihydrolase pathway.
Mots-clé
Crossing Over, Genetic, Gene Frequency, Mutation, Ornithine Carbamoyltransferase/genetics, Ornithine Carbamoyltransferase/metabolism, Pseudomonas aeruginosa/enzymology, Pseudomonas aeruginosa/genetics, Recombination, Genetic, Species Specificity, Transduction, Genetic
Pubmed
Web of science
Création de la notice
04/03/2008 18:01
Dernière modification de la notice
03/03/2018 19:53
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