Function and molecular architecture of the Yersinia injectisome tip complex.

Details

Serval ID
serval:BIB_9A8CE65AE586
Type
Article: article from journal or magazin.
Collection
Publications
Title
Function and molecular architecture of the Yersinia injectisome tip complex.
Journal
Molecular Microbiology
Author(s)
Broz P., Mueller C.A., Müller S.A., Philippsen A., Sorg I., Engel A., Cornelis G.R.
ISSN
0950-382X (Print)
ISSN-L
0950-382X
Publication state
Published
Issued date
2007
Peer-reviewed
Oui
Volume
65
Number
5
Pages
1311-1320
Language
english
Abstract
By quantitative immunoblot analyses and scanning transmission electron microscopy (STEM), we determined that the needle of the Yersinia enterocolitica E40 injectisome consists of 139 +/- 19 YscF subunits and that the tip complex is formed by three to five LcrV monomers. A pentamer represented the best fit for an atomic model of this complex. The N-terminal globular domain of LcrV forms the base of the tip complex, while the central globular domain forms the head. Hybrids between LcrV and its orthologues PcrV (Pseudomonas aeruginosa) or AcrV (Aeromonas salmonicida) were engineered and recombinant Y. enterocolitica expressing the different hybrids were tested for their capacity to form the translocation pore by a haemolysis assay. There was a good correlation between haemolysis, insertion of YopB into erythrocyte membranes and interaction between YopB and the N-terminal globular domain of the tip complex subunit. Hence, the base of the tip complex appears to be critical for the functional insertion of YopB into the host cell membrane.

Keywords
Antigens, Bacterial/chemistry, Antigens, Bacterial/genetics, Antigens, Bacterial/metabolism, Bacterial Proteins/chemistry, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Models, Molecular, Multiprotein Complexes, Pore Forming Cytotoxic Proteins/chemistry, Pore Forming Cytotoxic Proteins/genetics, Pore Forming Cytotoxic Proteins/metabolism, Protein Structure, Quaternary, Protein Subunits/chemistry, Protein Subunits/genetics, Protein Subunits/metabolism, Recombinant Fusion Proteins/chemistry, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/metabolism, Yersinia Infections/metabolism, Yersinia enterocolitica/pathogenicity, Yersinia enterocolitica/ultrastructure
Pubmed
Web of science
Open Access
Yes
Create date
25/10/2017 11:05
Last modification date
20/08/2019 16:01
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