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Anti-CD2 receptor antibodies activate the HIV long terminal repeat in T lymphocytes
Journal of Immunology
Journal Article --- Old month value: Oct 1
The CD2 T lymphocyte glycoprotein surface molecule mediates both cell to cell adhesion and T cell activation, two processes that are involved in the spread of HIV infection. Treatment of chronically HIV-infected PBMC with anti-CD2 mAb has been shown to induce the expression of infectious virus from these cultures. In this study we investigated the mechanisms whereby anti-CD2 antibodies stimulate viral production. We demonstrate that treatment of transiently transfected T lymphocytes with anti-CD2 antibodies results in activation of the HIV long terminal repeat. Furthermore, CAT assays using mutated HIV long terminal repeat-CAT constructs and gel shift assays demonstrate that this activation is dependent on the NF-kappa B enhancer. These studies suggest that interaction of CD2 with its natural ligand, LFA-3, may play a role in regulation of HIV expression.
Antibodies, Monoclonal/*immunology Antigens, CD2 Antigens, Differentiation, T-Lymphocyte/immunology/*physiology Enhancer Elements (Genetics) *Gene Expression Regulation, Viral *HIV Long Terminal Repeat Humans NF-kappa B/physiology Receptors, Immunologic/immunology/*physiology T-Lymphocytes/*microbiology Transfection Tumor Necrosis Factor-alpha/biosynthesis
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