Subpopulations of mature murine thymocytes: properties of CD4-CD8+ and CD4+CD8- thymocytes lacking the heat-stable antigen.

Détails

ID Serval
serval:BIB_98CA2BB8E2C4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Subpopulations of mature murine thymocytes: properties of CD4-CD8+ and CD4+CD8- thymocytes lacking the heat-stable antigen.
Périodique
Cellular Immunology
Auteur(s)
Wilson A., Day L.M., Scollay R., Shortman K.
ISSN
0008-8749 (Print)
ISSN-L
0008-8749
Statut éditorial
Publié
Date de publication
1988
Volume
117
Numéro
2
Pages
312-326
Langue
anglais
Résumé
The heat-stable antigen (HSA), recognized by the monoclonal antibodies M1/69, B2A2, and J11d, is low or absent on the surface of most murine peripheral T cells but present on all but 3% of thymocytes. The CD4-CD8+ and CD4+CD8- or "single positive" thymic populations may be divided into further subgroups based on surface HSA expression. One group, CD4-CD8+ and expressing very high levels of HSA (HSA++), is an immature, T cell antigen receptor (TcR) negative, outer cortical blast cell. However, a further subdivision of CD4-CD8+ and CD4+CD8- single positives may be made, into those negative to low for HSA (HSA-) and those expressing moderate amounts of HSA (HSA+). The proportion of HSA- single positives is low in the thymus of young mice, whereas the proportion of HSA+ single positives is similar to that of the adult. Both the HSA- and the HSA+ subsets of single positive thymocytes from adult mice are CD3+ and express the normal peripheral T cell incidence of V beta 8 determinants on the TcR. On stimulation with concanavalin A in limit-dilution culture both HSA- and HSA+ subsets of single positive thymocytes give a high frequency of proliferating clones, and the clones from both HSA- and HSA+ subsets of CD4-CD8+ thymocytes are cytotoxic. Thus both HSA- and HSA+ single positive thymocytes are functionally mature. The HSA- subsets of single positive thymocytes differ from the HSA+ subsets in being slightly larger in size, in expressing higher levels of MEL-14, in binding more peanut agglutinin, and in including a proportion of cells expressing high levels of the Pgp-1 glycoprotein. It is suggested that HSA- CD4-CD8+ and HSA- CD4+CD8- thymocytes are more mature than their HSA+ counterparts, and might represent a previously activated or "memory" thymic subpopulation.
Mots-clé
Animals, Antibodies, Monoclonal/diagnostic use, Antigens, Differentiation, T-Lymphocyte/analysis, Antigens, Differentiation, T-Lymphocyte/biosynthesis, Antigens, Surface/biosynthesis, Antigens, Surface/physiology, Cell Differentiation, Cell Survival, Complement System Proteins/immunology, Flow Cytometry, Fluorescent Antibody Technique, Hot Temperature, Male, Mice, Mice, Inbred CBA, Receptors, Antigen, T-Cell/biosynthesis, T-Lymphocytes/classification, T-Lymphocytes/cytology, T-Lymphocytes, Helper-Inducer/classification, T-Lymphocytes, Helper-Inducer/immunology, T-Lymphocytes, Regulatory/classification, T-Lymphocytes, Regulatory/immunology
Pubmed
Web of science
Création de la notice
11/04/2013 10:31
Dernière modification de la notice
20/08/2019 15:00
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