Calf rennet lysozyme

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_98A461BD5DAA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Calf rennet lysozyme
Périodique
Biochemical Journal
Auteur(s)
Pahud J.J., Widmer F.
ISSN
0264-6021
Statut éditorial
Publié
Date de publication
1982
Peer-reviewed
Oui
Volume
201
Numéro
3
Pages
661-664
Langue
anglais
Résumé
A glycosidase displaying endo-N-acetylmuramoylhydrolase specificity (EC 3.2.1.17) was isolated from calf rennet. This lysozyme was also present in abomasal secretions from calf and adult cattle. Multiple molecular forms revealed by electrofocusing might be artefacts. The main enzyme form had Mr approx. 15 000, pH optimum 5.0, pI7.5, and a remarkable conformation stability. Competitive inhibition was observed with both N-acetylglucosamine and N-acetylmuramic acid, with apparent Ki values of 29 mM and 2.4 mM respectively. The isolated enzyme also displayed significant chitinase activity.
Mots-clé
Animals, Cattle, Chymosin/*analysis, Hydrogen-Ion Concentration, Isoenzymes/isolation & purification, Kinetics, Muramidase/isolation & purification/*metabolism, Protein Conformation
Pubmed
Web of science
Création de la notice
13/08/2015 8:01
Dernière modification de la notice
20/08/2019 15:00
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