Porcine T-cell receptors: molecular and biochemical characterization.

Details

Serval ID
serval:BIB_97B2702FF103
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Porcine T-cell receptors: molecular and biochemical characterization.
Journal
Veterinary Immunology and Immunopathology
Author(s)
Thome M., Hirt W., Pfaff E., Reddehase M.J., Saalmüller A.
ISSN
0165-2427
Publication state
Published
Issued date
10/1994
Peer-reviewed
Oui
Volume
43
Number
1-3
Pages
13-18
Language
english
Notes
Publication types: Journal Article ; Review Publication Status: ppublish
Abstract
Two subclasses of CD3 associated T-cell receptors (TcR) have been described so far, consisting of either an alpha and beta chain (TcR alpha beta) or a gamma and delta chain (TcR gamma delta). Of the two subclasses, the TcR alpha beta is the one predominantly expressed on peripheral T lymphocytes of humans and rodents. TcR gamma delta T lymphocytes represent only a minor subset in these species. Among all mammalian species studied so far, swine showed the most diversified composition of the T-lymphocyte population characterized by the expression of CD4 and CD8 differentiation antigens. Besides CD4+CD8- and CD4-CD8+ T lymphocytes, CD4+CD8+ and CD4-CD8- T lymphocytes are prominent in the extrathymic T-lymphocyte compartment. Because of the lack of specific monoclonal antibodies (mAb), to date the porcine TcR can only be characterized with biochemical and molecular biological methods. TcR on porcine peripheral blood T lymphocytes with the phenotype CD4+ and/or CD8+ are characterized as 46-48 kDaR heterodimers which were supposed to represent the porcine TcR alpha beta. Biochemical analyses of the CD4-CD8- T lymphocytes revealed three distinct TcR gamma delta; all are characterized by a 40 kDa delta chain but differed in their gamma chains. One gamma chain with a molecular mass of 38 kDaR is preferentially expressed on CD4-CD8- T lymphocytes derived from peripheral blood; another chain with molecular mass of 37 kDaR is evenly distributed between CD4-CD8- T lymphocytes from blood and lymphoid tissues.(ABSTRACT TRUNCATED AT 250 WORDS)
Keywords
Amino Acid Sequence, Animals, Antigens, CD3/immunology, CD4-Positive T-Lymphocytes/immunology, CD8-Positive T-Lymphocytes/immunology, Immunophenotyping, Molecular Sequence Data, Receptors, Antigen, T-Cell, alpha-beta/genetics, Receptors, Antigen, T-Cell, alpha-beta/immunology, Receptors, Antigen, T-Cell, gamma-delta/genetics, Receptors, Antigen, T-Cell, gamma-delta/immunology, Swine/immunology
Pubmed
Web of science
Create date
24/01/2008 16:11
Last modification date
20/08/2019 15:59
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