The ABCG transporter PEC1/ABCG32 is required for the formation of the developing leaf cuticle in Arabidopsis.

Détails

ID Serval
serval:BIB_977630E5016E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The ABCG transporter PEC1/ABCG32 is required for the formation of the developing leaf cuticle in Arabidopsis.
Périodique
New Phytologist
Auteur(s)
Fabre G., Garroum I., Mazurek S., Daraspe J., Mucciolo A., Sankar M., Humbel B.M., Nawrath C.
ISSN
1469-8137 (Electronic)
ISSN-L
0028-646X
Statut éditorial
Publié
Date de publication
2016
Volume
209
Numéro
1
Pages
192-201
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The cuticle is an essential diffusion barrier on aerial surfaces of land plants whose structural component is the polyester cutin. The PERMEABLE CUTICLE1/ABCG32 (PEC1) transporter is involved in plant cuticle formation in Arabidopsis. The gpat6 pec1 and gpat4 gapt8 pec1 double and triple mutants are characterized. Their PEC1-specific contributions to aliphatic cutin composition and cuticle formation during plant development are revealed by gas chromatography/mass spectrometry and Fourier-transform infrared spectroscopy. The composition of cutin changes during rosette leaf expansion in Arabidopsis. C16:0 monomers are in higher abundance in expanding than in fully expanded leaves. The atypical cutin monomer C18:2 dicarboxylic acid is more prominent in fully expanded leaves. Findings point to differences in the regulation of several pathways of cutin precursor synthesis. PEC1 plays an essential role during expansion of the rosette leaf cuticle. The reduction of C16 monomers in the pec1 mutant during leaf expansion is unlikely to cause permeability of the leaf cuticle because the gpat6 mutant with even fewer C16:0 monomers forms a functional rosette leaf cuticle at all stages of development. PEC1/ABCG32 transport activity affects cutin composition and cuticle structure in a specific and non-redundant fashion.
Mots-clé
ATP-Binding Cassette Transporters/genetics, ATP-Binding Cassette Transporters/metabolism, Arabidopsis/genetics, Arabidopsis/growth & development, Flowers/genetics, Flowers/growth & development, Gene Expression Regulation, Plant, Gene Knockout Techniques, Membrane Lipids/metabolism, Mutation, Permeability, Plant Epidermis/genetics, Plant Epidermis/growth & development, Plant Leaves/genetics, Plant Leaves/growth & development, Plants, Genetically Modified
Pubmed
Web of science
Open Access
Oui
Création de la notice
15/01/2016 8:23
Dernière modification de la notice
20/08/2019 14:59
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