RNA polymerase III transcription from the human U6 and adenovirus type 2 VAI promoters has different requirements for human BRF, a subunit of human TFIIIB.

Détails

ID Serval
serval:BIB_9764BABC1FEC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
RNA polymerase III transcription from the human U6 and adenovirus type 2 VAI promoters has different requirements for human BRF, a subunit of human TFIIIB.
Périodique
Molecular and Cellular Biology
Auteur(s)
Mital R., Kobayashi R., Hernandez N.
ISSN
0270-7306[print], 0270-7306[linking]
Statut éditorial
Publié
Date de publication
12/1996
Volume
16
Numéro
12
Pages
7031-7042
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
Mammalian TFIIIB can be separated into two fractions required for transcription of the adenovirus type 2 VAI gene, which have been designated 0.38M-TFIIIB and 0.48M-TFIIIB. While 0.48M-TFIIIB has not been characterized, 0.38M-TFIIIB corresponds to a TBP-containing complex. We describe here the purification of this complex, which consists of TBP and a closely associated polypeptide of 88 kDa, and the isolation of a cDNA corresponding to the 88-kDa polypeptide. The predicted protein sequence reveals that the 88-kDa polypeptide corresponds to a human homolog of the Saccharomyces cerevisiae BRF protein, a subunit of yeast TFIIIB. Human BRF (hBRF) probably corresponds to TFIIIB90, a protein previously cloned by Wang and Roeder (Proc. Natl. Acad. Sci. USA 92:7026-7030, 1995), although its predicted amino acid sequence differs from that reported for TFIIIB90 over a stretch of 67 amino acids as a result of frameshifts. Immunodepletion of more than 90 to 95% of the hBRF present in a transcription extract severely debilitates transcription from the tRNA-type VAI promoter but does not affect transcription from the TATA box-containing human U6 promoter, suggesting that the 0.38M-TFIIIB complex, and perhaps hBRF as well, is not required for U6 transcription.
Mots-clé
Adenoviruses, Human/genetics, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Gene Expression Regulation, Genes, Viral, Humans, Molecular Sequence Data, Promoter Regions, Genetic/genetics, RNA Polymerase III/genetics, RNA, Viral/genetics, Ribonucleoprotein, U4-U6 Small Nuclear/genetics, Saccharomyces cerevisiae Proteins, Sequence Alignment, TATA-Binding Protein Associated Factors, Transcription Factor TFIIIB, Transcription Factors/genetics, Transcription Factors/isolation &amp, purification, Transcription, Genetic
Pubmed
Web of science
Création de la notice
21/01/2008 17:33
Dernière modification de la notice
20/08/2019 15:59
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