Use of human MAR elements to improve retroviral vector production.

Détails

ID Serval
serval:BIB_970D20965719
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Use of human MAR elements to improve retroviral vector production.
Périodique
Gene Therapy
Auteur(s)
Buceta M., Galbete J.L., Kostic C., Arsenijevic Y., Mermod N.
ISSN
1476-5462 (Electronic)
ISSN-L
0969-7128
Statut éditorial
Publié
Date de publication
2011
Peer-reviewed
Oui
Volume
18
Numéro
1
Pages
7-13
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Retroviral vectors have many favorable properties for gene therapies, but their use remains limited by safety concerns and/or by relatively lower titers for some of the safer self-inactivating (SIN) derivatives. In this study, we evaluated whether increased production of SIN retroviral vectors can be achieved from the use of matrix attachment region (MAR) epigenetic regulators. Two MAR elements of human origin were found to increase and to stabilize the expression of the green fluorescent protein transgene in stably transfected HEK-293 packaging cells. Introduction of one of these MAR elements in retroviral vector-producing plasmids yielded higher expression of the viral vector RNA. Consistently, viral titers obtained from transient transfection of MAR-containing plasmids were increased up to sixfold as compared with the parental construct, when evaluated in different packaging cell systems and transfection conditions. Thus, use of MAR elements opens new perspectives for the efficient generation of gene therapy vectors.
Mots-clé
Cells, Cultured, Gene Dosage, Genetic Vectors/genetics, Humans, Matrix Attachment Regions/genetics, Retroviridae/genetics, Transfection, Transgenes
Pubmed
Web of science
Open Access
Oui
Création de la notice
13/10/2010 15:15
Dernière modification de la notice
20/08/2019 14:59
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