Structural homology of human complement component C8 gamma and plasma protein HC: identity of the cysteine bond pattern

Détails

ID Serval
serval:BIB_96DB6EBBFD36
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Structural homology of human complement component C8 gamma and plasma protein HC: identity of the cysteine bond pattern
Périodique
Biochemical and Biophysical Research Communications
Auteur(s)
Haefliger  J. A., Jenne  D., Stanley  K. K., Tschopp  J.
ISSN
0006-291X (Print)
Statut éditorial
Publié
Date de publication
12/1987
Volume
149
Numéro
2
Pages
750-4
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec 16
Résumé
Anti-C8 alpha-gamma specific antibodies were used to isolate cDNA clones from a human liver expression library. Antibodies affinity-purified on the expressed hybrid protein of one clone bound exclusively to the gamma-chain of reduced C8 alpha-gamma. This clone, as well as a second full length cDNA clone obtained by hybridization screening, were sequenced and the complete primary structure for C8 gamma was established. Cyanogen bromide cleavage of C8 alpha-gamma released a 12 kDa carboxy-terminal C8 gamma fragment under both reducing and nonreducing conditions which was identified by fragment-specific, affinity-purified antibodies. Our data clearly show that C8 gamma has one internal disulfide bridge between cys-76 and cys-168 within the carboxy-terminal 12 kDa fragment, whereas the remaining cysteine residue 40 forms the disulfide bridge with C8 alpha. The overall sequence homology to plasma protein HC (23% amino acid identities) and the conservation of one internal cysteine bond and one free, surface-located cysteine residue suggests a highly conserved three-dimensional structure of C8 gamma and protein HC and also a possible functional relationship between these proteins.
Mots-clé
Alpha-Globulins/*analysis Amino Acid Sequence Base Sequence Complement C8/*analysis/genetics Cysteine DNA/analysis Humans Molecular Sequence Data
Pubmed
Web of science
Création de la notice
24/01/2008 16:19
Dernière modification de la notice
20/08/2019 15:58
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