The bZIP transcription factor Rca1p is a central regulator of a novel CO₂ sensing pathway in yeast.

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Version: author
Serval ID
serval:BIB_96470A19BDB8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The bZIP transcription factor Rca1p is a central regulator of a novel CO₂ sensing pathway in yeast.
Journal
Plos Pathogens
Author(s)
Cottier F., Raymond M., Kurzai O., Bolstad M., Leewattanapasuk W., Jiménez-López C., Lorenz M.C., Sanglard D., Váchová L., Pavelka N., Palková Z., Mühlschlegel F.A.
ISSN
1553-7374 (Electronic)
ISSN-L
1553-7366
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
8
Number
1
Pages
e1002485
Language
english
Notes
Publication types: Journal Article
Abstract
Like many organisms the fungal pathogen Candida albicans senses changes in the environmental CO(2) concentration. This response involves two major proteins: adenylyl cyclase and carbonic anhydrase (CA). Here, we demonstrate that CA expression is tightly controlled by the availability of CO(2) and identify the bZIP transcription factor Rca1p as the first CO(2) regulator of CA expression in yeast. We show that Rca1p upregulates CA expression during contact with mammalian phagocytes and demonstrate that serine 124 is critical for Rca1p signaling, which occurs independently of adenylyl cyclase. ChIP-chip analysis and the identification of Rca1p orthologs in the model yeast Saccharomyces cerevisiae (Cst6p) point to the broad significance of this novel pathway in fungi. By using advanced microscopy we visualize for the first time the impact of CO(2) build-up on gene expression in entire fungal populations with an exceptional level of detail. Our results present the bZIP protein Rca1p as the first fungal regulator of carbonic anhydrase, and reveal the existence of an adenylyl cyclase independent CO(2) sensing pathway in yeast. Rca1p appears to regulate cellular metabolism in response to CO(2) availability in environments as diverse as the phagosome, yeast communities or liquid culture.
Pubmed
Web of science
Open Access
Yes
Create date
24/03/2012 10:42
Last modification date
20/08/2019 14:58
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