Article: article from journal or magazin.
Cloning of an interferon-gamma regulated human melanoma-associated antigen: identity to the intercellular adhesion molecule ICAM-1.
The human melanoma-associated antigen identified by the monoclonal antibody (mAb) Me14-D12 is a cell surface protein whose expression is induced by interferon-gamma (IFN-gamma). We have recently reported the molecular cloning of a genomic probe specific for the gene and mRNA of this protein. By screening with the genomic probe, we have now isolated a full length 3.0 kb cDNA from a Raji cell line-derived lambda-gt10 library. Sequence analysis of this cDNA showed a 99.8% homology with the intercellular adhesion molecule-1 (ICAM-1). Mouse Ltk- cells stably transfected with the human cDNA clone were found to express the ICAM-1 antigenic determinants detected by mAb Me14-D12 and a reference anti-ICAM-1 mAb, as judged by surface immunofluorescence. Immunoprecipitation of surface-iodinated proteins with mAb Me14-D12 revealed the presence of a 90 kD molecule with identical mobility to ICAM-1. In addition, mAb Me14-D12 could inhibit the phorbolester-stimulated aggregation of U937 cells. The findings show that the human melanoma-associated Me14-D12 antigen is the adhesion molecule ICAM-1.
Antibodies, Monoclonal/immunology, Antibody Specificity, Antigens, Neoplasm, Base Sequence, Cell Adhesion Molecules/biosynthesis, Cell Adhesion Molecules/genetics, Cloning, Molecular, DNA/genetics, Gene Expression Regulation, Neoplastic/drug effects, Humans, Intercellular Adhesion Molecule-1, Interferon-gamma/pharmacology, Melanoma/immunology, Melanoma-Specific Antigens, Molecular Sequence Data, Neoplasm Proteins/biosynthesis, Neoplasm Proteins/genetics, Recombinant Fusion Proteins/biosynthesis, Sequence Homology, Nucleic Acid
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