Pulmonary Delivery of Virosome-Bound Antigen Enhances Antigen-Specific CD4(+) T Cell Proliferation Compared to Liposome-Bound or Soluble Antigen

Details

Serval ID
serval:BIB_959F2E06F10B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Pulmonary Delivery of Virosome-Bound Antigen Enhances Antigen-Specific CD4(+) T Cell Proliferation Compared to Liposome-Bound or Soluble Antigen
Journal
Front Immunol
Author(s)
Blom R. A. M., Amacker M., van Dijk R. M., Moser C., Stumbles P. A., Blank F., von Garnier C.
ISSN
1664-3224 (Print)
ISSN-L
1664-3224
Publication state
Published
Issued date
2017
Volume
8
Pages
359
Language
english
Notes
Blom, Rebecca A M
Amacker, Mario
van Dijk, R Maarten
Moser, Christian
Stumbles, Philip A
Blank, Fabian
von Garnier, Christophe
eng
Switzerland
Front Immunol. 2017 Apr 7;8:359. doi: 10.3389/fimmu.2017.00359. eCollection 2017.
Abstract
Pulmonary administration of biomimetic nanoparticles loaded with antigen may represent an effective strategy to directly modulate adaptive immune responses in the respiratory tract. Depending on the design, virosomes may not only serve as biomimetic antigen carriers but are also endowed with intrinsic immune-stimulatory properties. We designed fluorescently labeled influenza-derived virosomes and liposome controls coupled to the model antigen ovalbumin to investigate uptake, phenotype changes, and antigen processing by antigen-presenting cells exposed to such particles in different respiratory tract compartments. Both virosomes and liposomes were captured by pulmonary macrophages and dendritic cells alike and induced activation in particle-bearing cells by upregulation of costimulatory markers such as CD40, CD80, CD86, PD-L1, PD-L2, and ICOS-L. Though antigen processing and accumulation of both coupled and soluble antigen was similar between virosomes and liposomes, only ovalbumin-coupled virosomes generated a strong antigen-specific CD4(+) T cell proliferation. Pulmonary administrated antigen-coupled virosomes therefore effectively induced adaptive immune responses and may be utilized in novel preventive or therapeutic approaches in the respiratory tract.
Keywords
dendritic cell, immune modulation, liposomes, macrophage, respiratory tract, virosomes, virus-like particle
Pubmed
Create date
15/04/2021 9:58
Last modification date
01/05/2021 5:33
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