Immunolocalization of neuropeptide Y in human pancreatic endocrine tumors.

Détails

ID Serval
serval:BIB_957C2CDB82B1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Immunolocalization of neuropeptide Y in human pancreatic endocrine tumors.
Périodique
Peptides
Auteur(s)
Waeber G., Hurlimann J., Nicod P., Grouzmann E.
ISSN
0196-9781
Statut éditorial
Publié
Date de publication
1995
Peer-reviewed
Oui
Volume
16
Numéro
5
Pages
921-6
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
Neuropeptide Y (NPY) is a 36 amino acid peptide known to inhibit glucose-stimulated insulin secretion. NPY has recently been shown to be synthetized within rat islets of Langerhans and to be secreted in a differentiated rat insulin-secreting cell line, and as to this date the localization of NPY in human endocrine pancreas has not been reported. As NPY shares high amino acid sequence homology with peptide YY (PYY) and pancreatic polypeptide (PP), the polyclonal antibodies raised against these peptides often cross-react with each other. To demonstrate the presence of NPY in the human endocrine pancreas, we used a highly specific monoclonal antibody raised against NPY and another against its C-flanking peptide (CPON). We studied three cases of hyperplasia of Langerhans islets and 11 cases of endocrine tumors of the pancreas. NPY and CPON were detected in all three cases of hyperplasia. For the 11 pancreatic tumors, five and nine of the tumors were positive for the antibodies NPY and CPON, respectively. The two negative tumors for CPON immunoreactivity were differentiated insulinomas, which showed no evidence of other hormonal secretion. In normal Langerhans islet, NPY and CPON immunoreactivities were colocalized in glucagon-producing cells (alpha-cells) and in a few insulin-secreting cell (beta-cells).(ABSTRACT TRUNCATED AT 250 WORDS)
Mots-clé
Adult, Aged, Animals, Antibodies, Carcinoid Tumor, Carcinoma, Cell Line, Female, Humans, Hyperplasia, Immunohistochemistry, Insulin, Insulinoma, Islets of Langerhans, Male, Middle Aged, Neuroendocrine Tumors, Neuropeptide Y, Pancreatic Hormones, Pancreatic Neoplasms, Rats
Pubmed
Web of science
Création de la notice
25/01/2008 15:00
Dernière modification de la notice
03/03/2018 19:40
Données d'usage