Electrophysiological recording from Drosophila taste sensilla.

Détails

ID Serval
serval:BIB_9525DE76977C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Electrophysiological recording from Drosophila taste sensilla.
Périodique
Cold Spring Harbor Protocols
Auteur(s)
Benton R., Dahanukar A.
ISSN
1559-6095 (Electronic)
Statut éditorial
Publié
Date de publication
2011
Volume
2011
Numéro
7
Pages
839-850
Langue
anglais
Résumé
INTRODUCTION The chemical senses smell and taste, detect and discriminate an enormous diversity of environmental stimuli and provide fascinating but challenging models to investigate how sensory cues are represented in the brain. Important stimulus-coding events occur in peripheral sensory neurons, which express specific combinations of chemosensory receptors with defined ligand-response profiles. These receptors convert ligand recognition into spatial and temporal patterns of neural activity that are transmitted to and interpreted in central brain regions. Drosophila provides an attractive model to study chemosensory coding, because it possesses relatively simple peripheral olfactory and gustatory systems that display many organizational parallels to those of vertebrates. Moreover, virtually all of the peripheral chemosensory neurons are easily accessible for physiological analysis, as they are exposed on the surface of sensory organs in specialized sensory hairs called sensilla. In recent years, improvements in microscopy and instrumentation for electrode manipulation have opened up the much smaller Drosophila system to electrophysiological techniques, powerfully complementing many years of molecular genetic studies. As with most electrophysiological methods, there is probably no substitute for learning this technique directly from a laboratory in which it is already established. This protocol describes the basics of setting up the electrophysiology rig and stimulus delivery device, sample preparation, and performing and analyzing recordings of stimulus-evoked activity from Drosophila taste sensilla.
Pubmed
Création de la notice
15/08/2011 9:22
Dernière modification de la notice
20/08/2019 14:57
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