Role of protein kinase G in nitric oxide- and cGMP-induced relaxation of newborn ovine pulmonary veins

Détails

ID Serval
serval:BIB_951456D516CC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Role of protein kinase G in nitric oxide- and cGMP-induced relaxation of newborn ovine pulmonary veins
Périodique
Journal of Applied Physiology
Auteur(s)
Gao  Y., Dhanakoti  S., Tolsa  J. F., Raj  J. U.
ISSN
8750-7587 (Print)
Statut éditorial
Publié
Date de publication
09/1999
Volume
87
Numéro
3
Pages
993-8
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Sep
Résumé
In a variety of systemic blood vessels, protein kinase G (PKG) plays a critical role in mediating relaxation induced by agents that elevate cGMP, such as nitric oxide. The role of PKG in nitric oxide- and cGMP-induced relaxation is less certain in the pulmonary circulation. In the present study, we examined the effects of inhibitors of PKG on the responses of isolated fourth-generation pulmonary veins of newborn lambs (10 +/- 1 days of age) to nitric oxide and cGMP. In vessels preconstricted with endothelin-1, nitric oxide and 8-bromo-cGMP (a cell-membrane-permeable cGMP analog) induced concentration-dependent relaxation. The relaxation was significantly attenuated by beta-phenyl-1, N(2)-etheno-8-bromoguanosine-3',5'-cyclic monophosphorothionate (Rp-8-Br-PET-cGMPS; a PKG inhibitor) and N-[2-(methylamino)ethyl]5-isoquinolinesulfonamide [H-8; an inhibitor of PKG and protein kinase A (PKA)] but was not affected by KT-5720 (a PKA inhibitor). Biochemical study showed that PKG activity in newborn ovine pulmonary veins was inhibited by 8-Br-PET-cGMPS and H-8 but not by KT-5720. PKA activity was not affected by 8-Br-PET-cGMPS but was inhibited by H-8 and KT-5720. These results suggest that PKG is involved in relaxation of pulmonary veins of newborn lambs induced by nitric oxide and cGMP.
Mots-clé
Animals Animals, Newborn/*physiology Cyclic AMP-Dependent Protein Kinases/metabolism Cyclic GMP/analogs & derivatives/*pharmacology Cyclic GMP-Dependent Protein Kinases Endothelin-1/pharmacology Female Male Muscle Relaxation/drug effects Muscle, Smooth, Vascular/*drug effects Nitric Oxide/pharmacology/*physiology Protein Kinase Inhibitors Protein Kinases/*physiology Pulmonary Veins/*drug effects Sheep
Pubmed
Web of science
Création de la notice
28/01/2008 14:52
Dernière modification de la notice
03/03/2018 19:39
Données d'usage