Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy.

Détails

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Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_94D873A596E0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy.
Périodique
Journal of Biomedical Optics
Auteur(s)
Boss D., Kühn J., Jourdain P., Depeursinge C., Magistretti P.J., Marquet P.
ISSN
1560-2281 (Electronic)
ISSN-L
1083-3668
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
18
Numéro
3
Pages
36007
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
ABSTRACT. A dual-wavelength digital holographic microscope to measure absolute volume of living cells is proposed. The optical setup allows us to reconstruct two quantitative phase contrast images at two different wavelengths from a single hologram acquisition. When adding the absorbing dye fast green FCF as a dispersive agent to the extracellular medium, cellular thickness can be univocally determined in the full field of view. In addition to the absolute cell volume, the method can be applied to derive important biophysical parameters of living cells including osmotic membrane water permeability coefficient and the integral intracellular refractive index (RI). Further, the RI of transmembrane flux can be determined giving an indication about the nature of transported solutes. The proposed method is applied to cultured human embryonic kidney cells, Chinese hamster ovary cells, human red blood cells, mouse cortical astrocytes, and neurons.
Pubmed
Web of science
Création de la notice
26/03/2013 10:38
Dernière modification de la notice
03/03/2018 19:38
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