Cytosolic pH is a second messenger for glucose and regulates the PKA pathway through V-ATPase.

Détails

ID Serval
serval:BIB_94D3C2CCD9A5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cytosolic pH is a second messenger for glucose and regulates the PKA pathway through V-ATPase.
Périodique
EMBO Journal
Auteur(s)
Dechant R., Binda M., Lee S.S., Pelet S., Winderickx J., Peter M.
ISSN
1460-2075 (Electronic)
ISSN-L
0261-4189
Statut éditorial
Publié
Date de publication
2010
Volume
29
Numéro
15
Pages
2515-2526
Langue
anglais
Résumé
Glucose is the preferred carbon source for most cell types and a major determinant of cell growth. In yeast and certain mammalian cells, glucose activates the cAMP-dependent protein kinase A (PKA), but the mechanisms of PKA activation remain unknown. Here, we identify cytosolic pH as a second messenger for glucose that mediates activation of the PKA pathway in yeast. We find that cytosolic pH is rapidly and reversibly regulated by glucose metabolism and identify the vacuolar ATPase (V-ATPase), a proton pump required for the acidification of vacuoles, as a sensor of cytosolic pH. V-ATPase assembly is regulated by cytosolic pH and is required for full activation of the PKA pathway in response to glucose, suggesting that it mediates, at least in part, the pH signal to PKA. Finally, V-ATPase is also regulated by glucose in the Min6 beta-cell line and contributes to PKA activation and insulin secretion. Thus, these data suggest a novel and potentially conserved glucose-sensing pathway and identify a mechanism how cytosolic pH can act as a signal to promote cell growth.
Mots-clé
Animals, Cell Line, Cyclic AMP-Dependent Protein Kinases/metabolism, Cytosol/metabolism, Glucose/metabolism, Hydrogen-Ion Concentration, Insulin/secretion, Insulin-Secreting Cells/secretion, Mice, Second Messenger Systems, Vacuolar Proton-Translocating ATPases/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/09/2012 15:44
Dernière modification de la notice
08/05/2019 22:15
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