Molecular mechanisms involved in T cell activation. III. The role of extracellular calcium in antigen-induced lymphokine production and interleukin 2-induced proliferation of cloned cytotoxic T lymphocytes
Details
Serval ID
serval:BIB_94A93D498BD6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Molecular mechanisms involved in T cell activation. III. The role of extracellular calcium in antigen-induced lymphokine production and interleukin 2-induced proliferation of cloned cytotoxic T lymphocytes
Journal
Journal of Immunology
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
02/1988
Volume
140
Number
3
Pages
921-7
Notes
Journal Article --- Old month value: Feb 1
Abstract
The role that extracellular calcium plays in activating resting cloned cytotoxic T lymphocytes (CTL) to proliferate and to produce lymphokines was examined. In these cells, stimulation with interleukin 2 (IL-2) induced a proliferative response without a concomitant production of macrophage-activating factor (MAF), whereas stimulation with antigen or lectin (in the absence of IL-2) induced MAF production but not proliferation. In the case of IL-2-induced proliferation, extracellular calcium was required to initiate proliferation as well as to prevent cellular arrest later in the G2 + M phase of the cell cycle. In MAF production extracellular calcium was required both to activate the phosphatidylinositol signal-transducing mechanism and to mobilize intracellular calcium in antigen- or lectin-stimulated cytotoxic T lymphocytes. Further, extracellular calcium was required for only 8 of the 18 hr of stimulation time which was needed to achieve maximal MAF production, indicating that both calcium-dependent and -independent events exist in the signal pathway. Additional experiments with calcium ionophores and activators of protein kinase C indicated that although both intracellular calcium mobilization and de novo protein phosphorylation are involved in MAF production, an optimal increase in the level of intracellular calcium by itself is insufficient to induce the production of this lymphokine.
Keywords
Animals
Calcium/*pharmacology
Cell Line
Clone Cells/immunology
Cytotoxicity, Immunologic/drug effects
Extracellular Space/*physiology
H-2 Antigens/immunology
Interleukin-2/*pharmacology
Interphase
*Lymphocyte Activation/drug effects
Lymphokines/*biosynthesis
Macrophage-Activating Factors
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
T-Lymphocytes, Cytotoxic/drug effects/*immunology/metabolism
Pubmed
Web of science
Create date
28/01/2008 12:14
Last modification date
20/08/2019 15:57