Properties of rat anterior pituitary vasopressin receptors: relation to adenylate cyclase and the effect of corticotropin-releasing factor

Détails

ID Serval
serval:BIB_94A3A6DBCC29
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Properties of rat anterior pituitary vasopressin receptors: relation to adenylate cyclase and the effect of corticotropin-releasing factor
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Gaillard  R. C., Schoenenberg  P., Favrod-Coune  C. A., Muller  A. F., Marie  J., Bockaert  J., Jard  S.
ISSN
0027-8424 (Print)
Statut éditorial
Publié
Date de publication
05/1984
Volume
81
Numéro
9
Pages
2907-11
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
Crude plasma membrane fractions were prepared from female Wistar rat anterior pituitaries. These fractions contained a single population of specific 3H-labeled [8-lysine]vasopressin [( 3H]vasopressin) binding sites with a dissociation of constant (Kd) of 8 +/- 2 X 10(-9) M and maximal binding capacity of 244 +/- 45 fmol/mg of protein. The Kd values for a series of vasopressin structural analogues with selective vasopressor or antidiuretic activities were determined together with the corresponding corticotropin-releasing activities (isolated perfused pituitary cells were used). A good correspondence was found between the two sets of values, suggesting that the detected vasopressin binding sites are the receptors involved in vasopressin-induced corticotropin release. The order of potency of these analogues for the binding to hypophysial receptors was similar to that found for the binding to the receptors involved in the vasopressor response. Corticotropin-releasing factor and angiotensin did not affect vasopressin binding to pituitary membranes. Median eminence extracts inhibited [3H]vasopressin binding with an efficiency very close to that expected from their vasopressin content. Corticotropin-releasing factor activated, and angiotensin inhibited, the adenylate cyclase activity of pituitary membranes. Under the same experimental conditions, vasopressin did not influence adenylate cyclase activity nor did it affect the corticotropin-releasing factor-induced activation. These data support the view that vasopressin is one component of the multifactorial regulation of corticotropin release and that it acts through a cAMP-independent pathway. The potentiation by vasopressin of corticotropin-releasing factor-induced cAMP accumulation in intact cells very likely proceeds through indirect mechanisms, which are not expressed in broken cell preparations.
Mots-clé
Adenylate Cyclase/*metabolism Angiotensin II/pharmacology Animals Corticotropin-Releasing Hormone/*pharmacology Female Pituitary Gland, Anterior/*physiology Rats Receptors, Cell Surface/drug effects/*physiology Receptors, Vasopressin Structure-Activity Relationship Vasopressins/pharmacology
Pubmed
Web of science
Création de la notice
15/02/2008 17:58
Dernière modification de la notice
03/03/2018 19:38
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