Silver-assisted laser desorption ionization for high spatial resolution imaging mass spectrometry of olefins from thin tissue sections.

Détails

ID Serval
serval:BIB_948F074BA63E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Silver-assisted laser desorption ionization for high spatial resolution imaging mass spectrometry of olefins from thin tissue sections.
Périodique
Analytical Chemistry
Auteur(s)
Dufresne M., Thomas A., Breault-Turcot J., Masson J.F., Chaurand P.
ISSN
1520-6882 (Electronic)
ISSN-L
0003-2700
Statut éditorial
Publié
Date de publication
2013
Volume
85
Numéro
6
Pages
3318-3324
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Silver has been demonstrated to be a powerful cationization agent in mass spectrometry (MS) for various olefinic species such as cholesterol and fatty acids. This work explores the utility of metallic silver sputtering on tissue sections for high resolution imaging mass spectrometry (IMS) of olefins by laser desorption ionization (LDI). For this purpose, sputtered silver coating thickness was optimized on an assorted selection of mouse and rat tissues including brain, kidney, liver, and testis. For mouse brain tissue section, the thickness was adjusted to 23 ± 2 nm of silver to prevent ion suppression effects associated with a higher cholesterol and lipid content. On all other tissues, a thickness of at 16 ± 2 nm provided the best desorption/ionization efficiency. Characterization of the species by MS/MS showed a wide variety of olefinic compounds allowing the IMS of different lipid classes including cholesterol, arachidonic acid, docosahexaenoic acid, and triacylglyceride 52:3. A range of spatial resolutions for IMS were investigated from 150 μm down to the high resolution cellular range at 5 μm. The applicability of direct on-tissue silver sputtering to LDI-IMS of cholesterol and other olefinic compounds presents a novel approach to improve the amount of information that can be obtained from tissue sections. This IMS strategy is thus of interest for providing new biological insights on the role of cholesterol and other olefins in physiological pathways or disease.
Mots-clé
Alkenes/analysis, Animals, Brain Chemistry, Kidney/chemistry, Mass Spectrometry/methods, Mice, Microtomy/methods, Rats, Silver/chemistry, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
Pubmed
Web of science
Création de la notice
21/01/2015 16:44
Dernière modification de la notice
20/08/2019 14:57
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