Isolation and characterization of Marek's disease virus (MDV) cDNAs mapping to the BamHI-I2, BamHI-Q2, and BamHI-L fragments of the MDV genome from lymphoblastoid cells transformed and persistently infected with MDV.

Détails

ID Serval
serval:BIB_94385EBB9D26
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Isolation and characterization of Marek's disease virus (MDV) cDNAs mapping to the BamHI-I2, BamHI-Q2, and BamHI-L fragments of the MDV genome from lymphoblastoid cells transformed and persistently infected with MDV.
Périodique
Virology
Auteur(s)
Peng Q., Zeng M., Bhuiyan Z.A., Ubukata E., Tanaka A., Nonoyama M., Shirazi Y.
ISSN
0042-6822 (Print)
ISSN-L
0042-6822
Statut éditorial
Publié
Date de publication
10/11/1995
Peer-reviewed
Oui
Volume
213
Numéro
2
Pages
590-599
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
We have isolated and sequenced two cDNAs of sizes 2674 and 677 bp from a cDNA library derived from MKT-1, a lymphoblastoid cell line transformed and latently infected with Marek's disease virus (MDV) using probes corresponding to the right-hand end of the BamHI-I2 fragment of the MDV genome. The larger cDNA clone represents an abundant transcript, which extends from the right-hand end of BamHI-I2 to the adjacent BamHI-Q2 and BamHI-L fragments of the MDV genome and contains the Meq (MDV Eco-Q) open reading frame. The smaller cDNA clone represents a spliced transcript containing the putative DNA binding domain of Meq as well as sequences in the BamHI-L region. We prepared a polyclonal antibody against part of the protein sequence of Meq and detected a 44-kDa protein in MKT-1 cells and in cells lytically infected with MDV. In addition, riboprobes corresponding to sequences specific to each cDNA as well as shared sequences between cDNAs detected a number of transcripts in cells either lytically or latently infected with MDV. Our results indicate that the Meq transcriptional unit extends to the BamHI-L fragment and that the transcripts mapping to the right-hand end of the BamHI-I2 and adjacent BamHI-Q2 and BamHI-L fragments are not preferentially expressed during latency.

Mots-clé
Amino Acid Sequence, Animals, Base Sequence, Cell Transformation, Viral, DNA Primers, DNA, Complementary/isolation & purification, DNA, Viral/genetics, DNA, Viral/isolation & purification, Deoxyribonuclease BamHI, Gene Library, Genome, Viral, Herpesvirus 2, Gallid/genetics, Herpesvirus 2, Gallid/physiology, Lymphocytes/virology, Molecular Sequence Data, RNA, Viral/genetics, Restriction Mapping, Transcription, Genetic, Tumor Cells, Cultured, Viral Proteins/analysis, Viral Proteins/genetics, Virus Latency
Pubmed
Web of science
Open Access
Oui
Création de la notice
01/03/2018 16:41
Dernière modification de la notice
08/05/2019 22:13
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