Epidermal growth factor binding and protein kinase C activities in human breast cancer cell lines: possible quantitative relationship

Détails

ID Serval
serval:BIB_93EFCFFE90BD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Epidermal growth factor binding and protein kinase C activities in human breast cancer cell lines: possible quantitative relationship
Périodique
Cancer Research
Auteur(s)
Fabbro  D., Kung  W., Roos  W., Regazzi  R., Eppenberger  U.
ISSN
0008-5472 (Print)
Statut éditorial
Publié
Date de publication
06/1986
Volume
46
Numéro
6
Pages
2720-5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Résumé
Quantitative polyacrylamide gel electrophoresis analysis of Ca2+, phospholipid-dependent protein kinase (PKC) of human mammary tumor cell lines (MCF-7, ZR-75, T-47-D, MDA-MB-231, BT-20, and HBL-100) revealed that 80% of the total cellular PKC resided in the cytosol. The tumor cells with no detectable levels of estrogen receptors (MDA-MB-231, HBL-100, and BT-20 cells) exhibited significantly larger (P less than 0.001) cytosolic PKC activities than those cells that contained estrogen receptors (MCF-7, T-47-D, and ZR-75 cells). In addition, in estrogen receptor-negative cell lines, relatively high levels of specific low-affinity (apparent Kd = 700 pM) epidermal growth factor (EGF) binding activities were found as compared with estrogen receptor-positive cells with significantly (P less than 0.001) lower levels of specific high-affinity (apparent Kd = 90 pM) EGT binding. A significant positive correlation (P less than 0.01) was observed between the number of EGF receptor (Rs = 0.50) and/or the EGF receptor dissociation constants (Rs = 0.78) with the cytosolic PKC activity levels. These data indicate that, in human breast cancer cells, a positive relationship may exist between PKC activity, estrogen, and EGF receptors.
Mots-clé
Breast Neoplasms/*analysis Cell Line Chromatography, DEAE-Cellulose Cytosol/enzymology Electrophoresis, Polyacrylamide Gel Epidermal Growth Factor/metabolism Female Humans Iodine Radioisotopes/diagnostic use Protein Kinase C/*analysis Receptor, Epidermal Growth Factor Receptors, Cell Surface/*analysis Receptors, Estrogen/analysis
Pubmed
Web of science
Création de la notice
24/01/2008 15:30
Dernière modification de la notice
03/03/2018 19:36
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