Formation of the 3' end of U1 snRNA is directed by a conserved sequence located downstream of the coding region.

Détails

ID Serval
serval:BIB_93AF71EACCDC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Formation of the 3' end of U1 snRNA is directed by a conserved sequence located downstream of the coding region.
Périodique
EMBO Journal
Auteur(s)
Hernandez N.
ISSN
0261-4189
Statut éditorial
Publié
Date de publication
07/1985
Peer-reviewed
Oui
Volume
4
Numéro
7
Pages
1827-1837
Langue
anglais
Résumé
U1 is a small non-polyadenylated nuclear RNA that is transcribed by RNA polymerase II and is known to play a role in mRNA splicing. The mature 3' end of U1 snRNA is formed in at least two steps. The first step generates precursors of U1 RNA with a few extra nucleotides at the 3' end; in the second step, these precursors are shortened to mature U1 RNA. Here, I have determined the sequences required for the first step. Human U1 genes with various deletions and substitutions near the 3' end of the coding region were constructed and introduced into HeLa cells by DNA transfection. The structure of the RNA synthesized during transient expression of the exogenous U1 gene was analyzed by S1 mapping. The results show that a 13 nucleotide sequence located downstream from the U1 coding region and conserved among U1, U2 and U3 genes of different species is the only sequence required to direct the first step in the formation of the 3' end of U1 snRNA.
Mots-clé
Animals, Base Sequence, Chromosome Deletion, DNA Restriction Enzymes, Genes, Hela Cells, Histones, Humans, Mutation, RNA, RNA Polymerase II, RNA Splicing, RNA, Small Nuclear, Species Specificity, Transcription, Genetic, Transfection
Pubmed
Web of science
Création de la notice
21/01/2008 17:33
Dernière modification de la notice
20/08/2019 15:56
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