Identification and isolation of two ascomycete fungi from spores of the arbuscular mycorrhizal fungus Scutellospora castanea

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Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_939360CAB60F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Identification and isolation of two ascomycete fungi from spores of the arbuscular mycorrhizal fungus Scutellospora castanea
Périodique
Applied and Environmental Microbiology
Auteur(s)
Hijri  M., Redecker  D., MacDonald-Petetot  J., Voigt  K., Wostemeyer  J., Sanders  I. R.
Statut éditorial
Publié
Date de publication
2002
Volume
68
Numéro
9
Pages
4567-4573
Notes
588TX APPL ENVIRON MICROBIOL
Résumé
Two filamentous fungi with different phenotypes were isolated from crushed healthy spores or perforated dead spores of the arbuscular mycorrhizal fungus (AMF) Scutellospora castanea. Based on comparative sequence analysis of 5.8S ribosomal DNA and internal transcribed spacer fragments one isolate obtained from perforated dead spores only was assigned to the genus Nectria and the second obtained from both healthy and dead spores was assigned to Leptosphaeria a genus that also contains pathogens of plants in the Brassicaceae. PCR and randomly amplified polymorphic DNA-PCR analyses however did not indicate similarities between pathogens and the isolate. The presence of the two isolates in both healthy spores and perforated dead spores of S. castanea was finally confirmed by transmission electron microscopy by using distinctive characteristics of the isolates and S. castanea. The role of this fungus in S. castanea spores remains unclear but the results serve as a strong warning that sequences obtained from apparently healthy AMF spores cannot be presumed to be of glomalean origin and that this could present problems for studies on AMF genes.
Création de la notice
19/11/2007 11:40
Dernière modification de la notice
03/03/2018 19:35
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