CD34/QBEND10 immunostaining in bone marrow biopsies: an additional parameter for the diagnosis and classification of myelodysplastic syndromes.

Détails

ID Serval
serval:BIB_932A16D1E926
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
CD34/QBEND10 immunostaining in bone marrow biopsies: an additional parameter for the diagnosis and classification of myelodysplastic syndromes.
Périodique
European Journal of Haematology
Auteur(s)
Baur A.S., Meugé-Moraw C., Schmidt P.M., Parlier V., Jotterand M., Delacrétaz F.
ISSN
0902-4441
Statut éditorial
Publié
Date de publication
02/2000
Peer-reviewed
Oui
Volume
64
Numéro
2
Pages
71-79
Langue
anglais
Notes
Publication types: Journal Article - Publication Status: ppublish
Résumé
CD34/QBEND10 immunostaining has been assessed in 150 bone marrow biopsies (BMB) including 91 myelodysplastic syndromes (MDS), 16 MDS-related AML, 25 reactive BMB, and 18 cases where RA could neither be established nor ruled out. All cases were reviewed and classified according to the clinical and morphological FAB criteria. The percentage of CD34-positive (CD34 +) hematopoietic cells and the number of clusters of CD34+ cells in 10 HPF were determined. In most cases the CD34+ cell count was similar to the blast percentage determined morphologically. In RA, however, not only typical blasts but also less immature hemopoietic cells lying morphologically between blasts and promyelocytes were stained with CD34. The CD34+ cell count and cluster values were significantly higher in RA than in BMB with reactive changes (p<0.0001 for both), in RAEB than in RA (p=0.0006 and p=0.0189, respectively), in RAEBt than in RAEB (p=0.0001 and p=0.0038), and in MDS-AML than in RAEBt (p<0.0001 and p=0.0007). Presence of CD34+ cell clusters in RA correlated with increased risk of progression of the disease. We conclude that CD34 immunostaining in BMB is a useful tool for distinguishing RA from other anemias, assessing blast percentage in MDS cases, classifying them according to FAB, and following their evolution.
Mots-clé
Adolescent, Adult, Aged, Aged, 80 and over, Anemia, Refractory/diagnosis, Anemia, Refractory/pathology, Anemia, Refractory, with Excess of Blasts/diagnosis, Anemia, Refractory, with Excess of Blasts/pathology, Antibodies, Monoclonal, Antigens, CD34/analysis, Biopsy, Bone Marrow/chemistry, Bone Marrow/pathology, Cell Count, Child, Child, Preschool, Female, Hematopoietic Stem Cells/immunology, Hematopoietic Stem Cells/pathology, Humans, Immunohistochemistry, Infant, Leukemia, Myelomonocytic, Chronic/diagnosis, Leukemia, Myelomonocytic, Chronic/pathology, Male, Middle Aged, Myelodysplastic Syndromes/classification, Myelodysplastic Syndromes/diagnosis
Pubmed
Web of science
Création de la notice
25/01/2008 15:18
Dernière modification de la notice
03/03/2018 19:34
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