Comparative effects of GLP-1-(7-36) amide, oxyntomodulin and glucagon on rabbit gastric parietal cell function.

Détails

ID Serval
serval:BIB_93183FE11D77
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Comparative effects of GLP-1-(7-36) amide, oxyntomodulin and glucagon on rabbit gastric parietal cell function.
Périodique
European Journal of Pharmacology
Auteur(s)
Gros L., Hollande F., Thorens B., Kervran A., Bataille D.
ISSN
0014-2999[print], 0014-2999[linking]
Statut éditorial
Publié
Date de publication
02/1995
Volume
288
Numéro
3
Pages
319-327
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article
Publication Status: ppublish
Résumé
We have investigated in vitro, the effects of glucagon-like peptide-1-(7-36) amide (GLP-1-(7-36) amide), oxyntomodulin and glucagon on two rabbit parietal cell-enriched fractions (F3, F3n), with parietal cell contents of 60% and 88%, respectively. Histamine (10(-5) M) stimulated [14C]aminopyrine accumulation to an amount of 850% in excess of the basal level, whereas GLP-1-(7-36) amide (10(-7) M) and oxyntomodulin (10(-6) M) induced increases of 50% and 30%, respectively. With a histamine concentration of 10(-6) M, [14C]aminopyrine accumulation was stimulated to 498% in excess of the basal level; GLP-1-(7-36) amide (10(-7) M) and oxyntomodulin (10(-7) M) induced increases of 18% and 15%, respectively. With these parameters, oxyntomodulin[19-37] and glucagon were without effect. Specific binding of [125I]GLP-1-(7-36) amide to parietal cell plasma membranes was inhibited dose-dependently by GLP-1-(7-36) amide, oxyntomodulin and glucagon with inhibitory concentrations of 0.25 nM, 65 nM and 800 nM, respectively. No specific binding of [125I]oxyntomodulin or [125I]glucagon was detectable. GLP-1-(7-36) amide receptor mRNA was only detected in parietal cell-enriched fractions. GLP-1-(7-36) amide, oxyntomodulin and glucagon stimulated parietal cell cAMP production to similar maximal levels with median values close to 0.28 nM, 10.5 nM and 331.7 nM, whereas oxyntomodulin[19-37] had no effect. The maximal cAMP production induced by GLP-1-(7-36) amide, oxyntomodulin or glucagon was additive to that induced by histamine.(ABSTRACT TRUNCATED AT 250 WORDS)
Mots-clé
Aminopyrine/metabolism, Animals, Blotting, Northern, Cyclic AMP/biosynthesis, Glucagon/pharmacology, Glucagon-Like Peptide 1, Glucagon-Like Peptides/pharmacology, Histamine/pharmacology, Oxyntomodulin, Parietal Cells, Gastric/drug effects, Parietal Cells, Gastric/metabolism, Peptide Fragments/pharmacology, RNA, Messenger/genetics, Rabbits, Somatostatin/pharmacology
Pubmed
Web of science
Création de la notice
24/01/2008 14:41
Dernière modification de la notice
03/03/2018 19:33
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