Local applications of GM-CSF induce the recruitment of immune cells in cervical low-grade squamous intraepithelial lesions.

Détails

ID Serval
serval:BIB_930252EF2383
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Local applications of GM-CSF induce the recruitment of immune cells in cervical low-grade squamous intraepithelial lesions.
Périodique
American Journal of Reproductive Immunology (new York, N.y. : 1989)
Auteur(s)
Hubert P., Doyen J., Capelle X., Arafa M., Renoux V., Bisig B., Seidel L., Evrard B., Bousarghin L., Gerday C., Boniver J., Foidart J.M., Delvenne P., Jacobs N.
ISSN
1600-0897 (Electronic)
ISSN-L
1046-7408
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
64
Numéro
2
Pages
126-136
Langue
anglais
Notes
Publication types: Clinical Trial, Phase I ; Journal Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
PROBLEM: Quantitative alterations of antigen-presenting cells (APC) in (pre)neoplastic lesions of the uterine cervix associated with human papillomavirus (HPV) infection suggest a diminished capacity to capture viral antigens and to induce a protective immune response.
METHOD OF STUDY: To test whether a cervical application of GM-CSF could restore an immune response against HPV in women with cervical low-grade squamous intraepithelial lesions (LSIL), we performed two clinical trials with 11 healthy women and 15 patients with LSIL.
RESULTS: GM-CSF applications were well tolerated in all enrolled women, and no difference in toxicity between the treated and placebo groups was observed during the follow-up (until 30 months). Interestingly, in the GM-CSF treated group, a significant increase of APC and cytotoxic T-lymphocyte infiltration was observed in the cervical biopsies with no change in regulatory T cell numbers. All the HPV16(+) patients exhibited an immune response against HPV16 after GM-CSF applications, as shown by NK and/or T cells producing IFN-gamma whereas no cellular immune response was observed before the treatment. Moreover, the anti-virus-like particles antibody titers also increased after the treatment.
CONCLUSION: These encouraging results obtained from a limited number of subjects justify further study on the therapeutic effect of APC in cervical (pre)neoplastic lesions.
Mots-clé
Adaptive Immunity/drug effects, Administration, Topical, Adult, Antibodies, Viral/blood, Antigen-Presenting Cells/drug effects, Antigen-Presenting Cells/immunology, Cell Line, Cell Proliferation/drug effects, Cervical Intraepithelial Neoplasia/drug therapy, Cervical Intraepithelial Neoplasia/immunology, Female, Granulocyte-Macrophage Colony-Stimulating Factor/administration & dosage, Granulocyte-Macrophage Colony-Stimulating Factor/adverse effects, Human papillomavirus 16/drug effects, Human papillomavirus 16/immunology, Humans, Immunologic Factors/administration & dosage, Immunologic Factors/adverse effects, Keratinocytes/drug effects, Lymphocyte Subsets/drug effects, Lymphocyte Subsets/immunology, Lymphocytes, Tumor-Infiltrating/drug effects, Lymphocytes, Tumor-Infiltrating/immunology, Middle Aged, Natural Killer T-Cells/drug effects, Natural Killer T-Cells/immunology, Neoplasms, Squamous Cell/drug therapy, Neoplasms, Squamous Cell/immunology, Papillomavirus Infections/blood, Papillomavirus Infections/complications, T-Lymphocytes, Cytotoxic/drug effects, T-Lymphocytes, Cytotoxic/immunology, Uterine Cervical Neoplasms/drug therapy, Uterine Cervical Neoplasms/immunology, Young Adult
Pubmed
Web of science
Création de la notice
20/09/2016 9:41
Dernière modification de la notice
03/03/2018 19:33
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