The Oct-1 POU domain activates snRNA gene transcription by contacting a region in the SNAPc largest subunit that bears sequence similarities to the Oct-1 coactivator OBF-1.

Détails

ID Serval
serval:BIB_92EBEDE9E5F9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The Oct-1 POU domain activates snRNA gene transcription by contacting a region in the SNAPc largest subunit that bears sequence similarities to the Oct-1 coactivator OBF-1.
Périodique
Genes and Development
Auteur(s)
Ford E., Strubin M., Hernandez N.
ISSN
0890-9369[print], 0890-9369[linking]
Statut éditorial
Publié
Date de publication
11/1998
Volume
12
Numéro
22
Pages
3528-3540
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
The RNA polymerases II and III snRNA gene promoters contain an octamer sequence as part of the enhancer and a proximal sequence element (PSE) as part of the core promoter. The octamer and the PSE bind the POU domain activator Oct-1 and the basal transcription factor SNAPc, respectively. Oct-1, but not Oct-1 with a single E7R mutation within the POU domain, binds cooperatively with SNAPc and, in effect, recruits SNAPc to the PSE. Here, we show that SNAPc recruitment is mediated by an interaction between the Oct-1 POU domain and a small region of the largest subunit of SNAPc, SNAP190. This SNAP190 region is strikingly similar to a region in the B-cell-specific Oct-1 coactivator, OBF-1, that is required for interaction with octamer-bound Oct-1 POU domain. The Oct-1 POU domain-SNAP190 interaction is a direct protein-protein contact as determined by the isolation of a switched specificity SNAP190 mutant that interacts with Oct-1 POU E7R but not with wild-type Oct-1 POU. We also show that this direct protein-protein contact results in activation of transcription. Thus, we have identified an activation target of a human activator, Oct-1, within its cognate basal transcription complex.
Mots-clé
Amino Acid Sequence, Binding Sites/genetics, DNA Probes/genetics, DNA Probes/metabolism, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Homeodomain Proteins/genetics, Host Cell Factor C1, Humans, Molecular Sequence Data, Mutation/genetics, Octamer Transcription Factor-1, Promoter Regions, Genetic/genetics, Protein Binding/genetics, RNA Polymerase II/genetics, RNA Polymerase III/genetics, RNA, Small Nuclear/genetics, Trans-Activators/genetics, Trans-Activators/metabolism, Transcription Factors/genetics, Transcription Factors/metabolism, Transcriptional Activation/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/01/2008 17:33
Dernière modification de la notice
20/08/2019 15:55
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