The Oct-1 POU domain activates snRNA gene transcription by contacting a region in the SNAPc largest subunit that bears sequence similarities to the Oct-1 coactivator OBF-1.

Details

Serval ID
serval:BIB_92EBEDE9E5F9
Type
Article: article from journal or magazin.
Collection
Publications
Title
The Oct-1 POU domain activates snRNA gene transcription by contacting a region in the SNAPc largest subunit that bears sequence similarities to the Oct-1 coactivator OBF-1.
Journal
Genes and Development
Author(s)
Ford E., Strubin M., Hernandez N.
ISSN
0890-9369[print], 0890-9369[linking]
Publication state
Published
Issued date
11/1998
Volume
12
Number
22
Pages
3528-3540
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Abstract
The RNA polymerases II and III snRNA gene promoters contain an octamer sequence as part of the enhancer and a proximal sequence element (PSE) as part of the core promoter. The octamer and the PSE bind the POU domain activator Oct-1 and the basal transcription factor SNAPc, respectively. Oct-1, but not Oct-1 with a single E7R mutation within the POU domain, binds cooperatively with SNAPc and, in effect, recruits SNAPc to the PSE. Here, we show that SNAPc recruitment is mediated by an interaction between the Oct-1 POU domain and a small region of the largest subunit of SNAPc, SNAP190. This SNAP190 region is strikingly similar to a region in the B-cell-specific Oct-1 coactivator, OBF-1, that is required for interaction with octamer-bound Oct-1 POU domain. The Oct-1 POU domain-SNAP190 interaction is a direct protein-protein contact as determined by the isolation of a switched specificity SNAP190 mutant that interacts with Oct-1 POU E7R but not with wild-type Oct-1 POU. We also show that this direct protein-protein contact results in activation of transcription. Thus, we have identified an activation target of a human activator, Oct-1, within its cognate basal transcription complex.
Keywords
Amino Acid Sequence, Binding Sites/genetics, DNA Probes/genetics, DNA Probes/metabolism, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Homeodomain Proteins/genetics, Host Cell Factor C1, Humans, Molecular Sequence Data, Mutation/genetics, Octamer Transcription Factor-1, Promoter Regions, Genetic/genetics, Protein Binding/genetics, RNA Polymerase II/genetics, RNA Polymerase III/genetics, RNA, Small Nuclear/genetics, Trans-Activators/genetics, Trans-Activators/metabolism, Transcription Factors/genetics, Transcription Factors/metabolism, Transcriptional Activation/genetics
Pubmed
Web of science
Open Access
Yes
Create date
21/01/2008 16:33
Last modification date
20/08/2019 14:55
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