Article: article from journal or magazin.
Presence of T 145 on cytolytic T cell lines and their lectin-resistant mutants.
European Journal of Immunology
Murine cytolytic T cell lines have been analyzed for the expression of two surface glycoproteins called T145 and T130. T145, known to be expressed by activated cytolytic T cells, is also expressed by such lines, but T130, which has been described by a universal T cell marker, is not. Our results suggest a structural relationship between T145 and T130. Vicia villosa lectin, which binds selectively to T145 of activated T cells and which is cytotoxic for cytolytic T cell lines, has been used to select lectin-resistant mutants from these lines. Five independent lectin-resistant mutants have been obtained. All of them are cytolytically active, bind up to 100-fold less lectin than the parental lines, but still express T145 or a closely related glycoprotein.
Animals, Binding Sites, Cell Line, Cytotoxicity, Immunologic, Electrophoresis, Polyacrylamide Gel, Glycoproteins, Lectins/pharmacology, Membrane Proteins/genetics, Membrane Proteins/immunology, Mice, Mice, Inbred AKR, Mice, Inbred BALB C, Mutation, T-Lymphocytes/immunology
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