Intranasal Vaccination With Salmonella-Derived Serodominant Secreted Effector Protein B Associated With Gas-Filled Microbubbles Partially Protects Against Gut Infection in Mice.

Details

Serval ID
serval:BIB_92738AAE6623
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Intranasal Vaccination With Salmonella-Derived Serodominant Secreted Effector Protein B Associated With Gas-Filled Microbubbles Partially Protects Against Gut Infection in Mice.
Journal
The Journal of infectious diseases
Author(s)
Pigny F., Lassus A., Terrettaz J., Tranquart F., Corthésy B., Bioley G.
ISSN
1537-6613 (Electronic)
ISSN-L
0022-1899
Publication state
Published
Issued date
01/08/2016
Peer-reviewed
Oui
Volume
214
Number
3
Pages
438-446
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Salmonella infection is an increasingly important public health problem owing to the emergence of multidrug resistance and the lack of broadly efficient vaccines. Novel strategies of vaccination are required to induce protective immune responses at mucosal surfaces and in the circulation, to limit bacteria entry and dissemination. To this aim, intranasal anti-Salmonella vaccination with an innovative formulation composed of gas-filled microbubbles and the pathogen-derived protective protein serodominant secreted effector protein B (SseB-MB) was evaluated in a mouse infection model. Intranasal application of SseB-MB induced gut and systemic immunoglobulin A, T-helper type 17 cell (Th17), and Th1 responses, all of which are associated with natural immunity against Salmonella In vaccinated mice, a significant reduction in bacterial load was observed in intestinal tissues and the spleen after an otherwise lethal oral infection. Therefore, MB serve as an efficient carrier for nasal delivery of a Salmonella antigen that results in protection upon activation of the common mucosal immune system.

Keywords
Administration, Intranasal, Animals, Antibodies, Bacterial/analysis, Antibodies, Bacterial/blood, Antigens, Bacterial/administration & dosage, Antigens, Bacterial/immunology, Bacterial Load, Bacterial Proteins/administration & dosage, Bacterial Proteins/immunology, Disease Models, Animal, Drug Carriers/administration & dosage, Female, Gastrointestinal Tract/immunology, Gastrointestinal Tract/microbiology, Immunity, Mucosal, Immunoglobulin A/analysis, Immunoglobulin A/blood, Mice, Inbred BALB C, Molecular Chaperones/administration & dosage, Molecular Chaperones/immunology, Salmonella/immunology, Salmonella Infections/immunology, Salmonella Infections/prevention & control, Salmonella Vaccines/administration & dosage, Salmonella Vaccines/immunology, Th1 Cells/immunology, Th17 Cells/immunology, Treatment Outcome, Salmonella, adjuvant, antigen carrier, gastrointestinal tract, intranasal vaccination, microbubbles, protection
Pubmed
Web of science
Open Access
Yes
Create date
11/12/2017 11:48
Last modification date
20/08/2019 14:55
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