Entry and transcription as key determinants of differences in CD4 T-cell permissiveness to human immunodeficiency virus type 1 infection.

Détails

ID Serval
serval:BIB_92680CCF2EA7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Entry and transcription as key determinants of differences in CD4 T-cell permissiveness to human immunodeficiency virus type 1 infection.
Périodique
Journal of virology
Auteur(s)
Ciuffi A., Bleiber G., Muñoz M., Martinez R., Loeuillet C., Rehr M., Fischer M., Günthard H.F., Oxenius A., Meylan P., Bonhoeffer S., Trono D., Telenti A.
ISSN
0022-538X
Statut éditorial
Publié
Date de publication
2004
Peer-reviewed
Oui
Volume
78
Numéro
19
Pages
10747-54
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
Isolated primary human cells from different donors vary in their permissiveness-the ability of cells to be infected and sustain the replication of human immunodeficiency virus type 1 (HIV-1). We used replicating HIV-1 and single-cycle lentivirus vectors in a population approach to identify polymorphic steps during viral replication. We found that phytohemagglutinin-stimulated CD4(+) CD45RO(+) CD57(-) T cells from healthy blood donors (n = 128) exhibited a 5.2-log-unit range in virus production. For 20 selected donors representing the spectrum of CD4 T-cell permissiveness, we could attribute up to 42% of the total variance in virus production to entry factors and 48% to postentry steps. Efficacy at key intracellular steps of the replicative cycle (reverse transcription, integration, transcription and splicing, translation, and budding and release) varied from 0.71 to 1.45 log units among donors. However, interindividual differences in transcription efficiency alone accounted for 64 to 83% of the total variance in virus production that was attributable to postentry factors. While vesicular stomatitis virus G protein-mediated fusion was more efficacious than CCR5/CD4 entry, the latter resulted in greater transcriptional activity per proviral copy. The phenotype of provirus transcription was stable over time, indicating that it represents a genetic trait.
Mots-clé
Antigens, CD4, Antigens, CD45, Antigens, CD57, Biological Transport, CD4-Positive T-Lymphocytes, Cells, Cultured, DNA, Viral, HIV Core Protein p24, HIV-1, Humans, Protein Biosynthesis, Protein Tyrosine Phosphatase, Non-Receptor Type 1, Proviruses, RNA Splicing, RNA, Viral, Receptors, CCR5, Time Factors, Transcription, Genetic, Vesicular stomatitis Indiana virus, Virus Integration, Virus Replication
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 15:46
Dernière modification de la notice
08/05/2019 22:06
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