Taxol and tau overexpression induced calpain-dependent degradation of the microtubule-destabilizing protein SCG10

Détails

Ressource 1Télécharger: BIB_924545418DFF.P001.pdf (1840.74 [Ko])
Etat: Serval
Version: Author's accepted manuscript
ID Serval
serval:BIB_924545418DFF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Taxol and tau overexpression induced calpain-dependent degradation of the microtubule-destabilizing protein SCG10
Périodique
Experimental Neurology
Auteur(s)
Vega I.E., Hamano T., Propost J.A., Grenningloh G., Yen S.H.
ISSN
0014-4886 (Print)
1090-2430 (Electronic)
ISSN-L
0014-4886
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
202
Numéro
1
Pages
152-160
Langue
anglais
Notes
Publication types: Article ; research-article Identifiant PubMed Central: PMC3696491
Résumé
Microtubule-stabilizing and -destabilizing proteins play a crucial role in regulating the dynamic instability of microtubules during neuronal development and synaptic transmission. The microtubule-destabilizing protein SCG10 is a neuron-specific protein implicated in neurite outgrowth. The SCG10 protein is significantly reduced in mature neurons, suggesting that its expression is developmentally regulated. In contrast, the microtubule-stabilizing protein tau is expressed in mature neurons and its function is essential for the maintenance of neuronal polarity and neuronal survival. Thus, the establishment and maintenance of neuronal polarity may down-regulate the protein level/function of SCG10. In this report, we show that treatment of PC12 cells and neuroblastoma cells with the microtubule-stabilizing drug Taxol induced a rapid degradation of the SCG10 protein. Consistently, overexpression of tau protein in neuroblastoma cells also induced a reduction in SCG10 protein levels. Calpain inhibitor MDL-28170, but not caspase inhibitors, blocked a significant decrease in SCG10 protein levels. Collectively, these results indicate that tau overexpression and Taxol treatment induced a calpain-dependent degradation of the microtubule-destabilizing protein SCG10. The results provide evidence for the existence of an intracellular mechanism involved in the regulation of SCG10 upon microtubule stabilization.
Mots-clé
Blotting, Western/methods, Calpain/metabolism, Cysteine Proteinase Inhibitors/pharmacology, Enzyme Activation/drug effects, Gene Expression Regulation/drug effects, Leupeptins/pharmacology, Membrane Proteins/metabolism, Paclitaxel/pharmacology, Transfection/methods, Tubulin Modulators/pharmacology, tau Proteins/metabolism
Pubmed
Web of science
Création de la notice
11/07/2016 11:04
Dernière modification de la notice
03/03/2018 19:31
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