Four novel members of the connexin family of gap junction proteins. Molecular cloning, expression, and chromosome mapping.

Détails

ID Serval
serval:BIB_922FB75C3F7D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Four novel members of the connexin family of gap junction proteins. Molecular cloning, expression, and chromosome mapping.
Périodique
Journal of Biological Chemistry
Auteur(s)
Haefliger J.A., Bruzzone R., Jenkins N.A., Gilbert D.J., Copeland N.G., Paul D.L.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
01/1992
Peer-reviewed
Oui
Volume
267
Numéro
3
Pages
2057-2064
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article
Résumé
We have used low stringency hybridization and polymerase chain reaction (PCR) amplification with degenerate oligonucleotides to identify four new members of the rat connexin gene family. On the basis of their predicted molecular mass, these proteins have been designated connexin (Cx) 40 (Cx40), Cx37, Cx33, and Cx31.1. The new connexins exhibit all of the conserved structural features of the connexin family, including highly similar extracellular and transmembrane domains but divergent major cytoplasmic domains. On the basis of primary sequence similarity, the connexin family may be divided into two classes. Cx40, Cx37, and Cx33 are similar to the previously characterized Cx43 and Cx46. Cx31.1 is similar to Cx26, Cx31, and Cx32. Cx37 and Cx40 mRNAs are expressed in a wide variety of adult organs and tissues, with particular abundance in lung. However, their relative levels are different in many organs and thus their distribution is not completely coincident. Cx33 and Cx31.1 genes exhibit a much more restricted pattern of expression; mRNAs are detected only in testes and skin, respectively. Chromosomal mapping studies indicate that Cx26 and Cx46 are tightly linked on chromosome 14, and Cx37 and Cx31.1 are linked on chromosome 4, while the rest of the connexin genes are dispersed.
Mots-clé
Amino Acid Sequence, Animals, Base Sequence, Blotting, Northern, Blotting, Southern, Chromosome Mapping, Cloning, Molecular/methods, Connexins, Crosses, Genetic, DNA/genetics, DNA/isolation & purification, Gene Expression, Genomic Library, Membrane Proteins/genetics, Models, Structural, Molecular Sequence Data, Multigene Family, Oligodeoxyribonucleotides, Polymerase Chain Reaction/methods, Protein Conformation, RNA/genetics, RNA/isolation & purification, Rats, Rats, Inbred Strains, Sequence Homology, Nucleic Acid
Pubmed
Web of science
Création de la notice
25/01/2008 14:48
Dernière modification de la notice
03/03/2018 19:31
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