Cutting edge: thymic crosstalk regulates delta-like 4 expression on cortical epithelial cells.

Détails

ID Serval
serval:BIB_921D7A955B99
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cutting edge: thymic crosstalk regulates delta-like 4 expression on cortical epithelial cells.
Périodique
Journal of Immunology
Auteur(s)
Fiorini E., Ferrero I., Merck E., Favre S., Pierres M., Luther S.A., MacDonald H.R.
ISSN
1550-6606[electronic]
Statut éditorial
Publié
Date de publication
2008
Volume
181
Numéro
12
Pages
8199-8203
Langue
anglais
Résumé
Interactions between Notch1 receptors on lymphoid progenitors and Delta-like 4 (DL4) ligands on cortical thymic epithelial cells (cTEC) are essential for T cell lineage commitment, expansion, and maturation in the thymus. Using a novel mAb against DL4, we show that DL4 levels on cTEC are very high in the fetal and neonatal thymus when thymocyte expansion is maximal but decrease dramatically in the adult when steady-state homeostasis is attained. Analysis of mutant mouse strains where thymocyte development is blocked at different stages indicates that lymphostromal interactions ("thymus crosstalk") are required for DL4 down-regulation on cTEC. Reconstitution of thymocyte development in these mutant mice further suggests that maturation of thymocytes to the CD4(+)CD8(+) stage and concomitant expansion are needed to promote DL4 down-regulation on cTEC. Collectively, our data support a model where thymic crosstalk quantitatively regulates the rate of Notch1-dependent thymopoiesis by controlling DL4 expression levels on cTEC.
Mots-clé
Animals, Animals, Newborn, Cell Communication/genetics, Cell Communication/immunology, Down-Regulation/immunology, Epithelial Cells/immunology, Epithelial Cells/metabolism, Intracellular Signaling Peptides and Proteins/antagonists & inhibitors, Intracellular Signaling Peptides and Proteins/genetics, Lymphopoiesis/genetics, Lymphopoiesis/immunology, Male, Membrane Proteins/antagonists & inhibitors, Membrane Proteins/biosynthesis, Mice, Mice, Inbred C57BL, Mice, Knockout, Models, Immunological, Rats, Receptor, Notch1/physiology, Stromal Cells/cytology, Stromal Cells/immunology, Thymus Gland/cytology, Thymus Gland/embryology
Pubmed
Web of science
Création de la notice
12/10/2009 15:59
Dernière modification de la notice
03/03/2018 19:30
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