Evidence for adenylyl cyclase-dependent receptors for parathyroid hormone (PTH)-related protein in rabbit kidney glomeruli.

Détails

ID Serval
serval:BIB_91CAC6B41A0E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Evidence for adenylyl cyclase-dependent receptors for parathyroid hormone (PTH)-related protein in rabbit kidney glomeruli.
Périodique
Life Sciences
Auteur(s)
Massfelder T., Saussine C., Simeoni U., Enanga B., Judes C., Helwig J.J.
ISSN
0024-3205 (Print)
ISSN-L
0024-3205
Statut éditorial
Publié
Date de publication
1993
Peer-reviewed
Oui
Volume
53
Numéro
11
Pages
875-881
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
Studies were conducted to test whether parathyroid hormone-related protein (PTHrP) is able to stimulate adenylate cyclase activity in isolated rabbit glomeruli. Maximal stimulations were reached at 10(-7) M of human PTHrP-(1-34) or rat PTH-(1-34) and showed a 3-3.3 fold increase over basal activity. The potency (EC50) values were close to 10(-9) M. The guanyl nucleotide GTP, at 10(-5) M, potentiated the effect of PTH and PTHrP but reduced their potency. The combined effect of maximal concentrations of PTHrP and PTH was not additive, and the PTH antagonist [Nle8.18, Tyr34]-bPTH-(3-34)amide inhibited both PTHrP- and PTH-stimulated adenylate cyclase activities. These findings suggest that PTHrP could affect glomerular function through changes in glomerular cAMP content by interaction with PTH receptors.
Mots-clé
Adenylate Cyclase/metabolism, Animals, Enzyme Activation, Kidney Glomerulus/enzymology, Kidney Glomerulus/metabolism, L-Lactate Dehydrogenase/metabolism, Parathyroid Hormone/metabolism, Parathyroid Hormone-Related Protein, Proteins/metabolism, Rabbits, Receptors, Cell Surface/metabolism, Receptors, Parathyroid Hormone
Pubmed
Web of science
Création de la notice
22/02/2015 11:05
Dernière modification de la notice
03/03/2018 19:29
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